GABRA1 Antikörper (AA 28-43)
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- Target Alle GABRA1 Antikörper anzeigen
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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Bindungsspezifität
- AA 28-43
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Reaktivität
- Ratte, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser GABRA1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Spezifität
- Specific for GABA-A receptor alpha1.
- Aufreinigung
- purified IgM. Azide was added before lyophilization.
- Immunogen
- Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Klon
- 53D3
- Isotyp
- IgM
- Top Product
- Discover our top product GABRA1 Primärantikörper
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- Applikationshinweise
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WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Kommentare
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ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS, 0.02% sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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-
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
: "
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How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
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- Target
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
- Andere Bezeichnung
- GABA-A Receptor Alpha1 (GABRA1 Produkte)
- Synonyme
- ECA4 antikoerper, EJM antikoerper, EJM5 antikoerper, zgc:153566 antikoerper, Gabra-1 antikoerper, gamma-aminobutyric acid type A receptor alpha1 subunit antikoerper, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antikoerper, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antikoerper, gamma-aminobutyric acid receptor subunit alpha-1 antikoerper, GABRA1 antikoerper, Gabra1 antikoerper, gabra1 antikoerper, LOC100653509 antikoerper
- Hintergrund
- Synonyms: GABA(A) R alpha1
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