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GFP Antikörper

Name: GFP Antikörper
SKU: ABIN1742293
Price: 492.46 EUR
Availability: InStock

GFP Reaktivität: Aequorea victoria WB, ICC Wirt: Kaninchen Polyclonal unconjugated

dilution: 1 : 1000, sample: total cell lysate of GFP transfected fibroblasts

Image no. 1 for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293)

2 Abbildungen

(1 reference)

Produktnummer ABIN1742293

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Reaktivität
178

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Wirt
74

73

35

11

9

6

5

1
Kaninchen
Passende Sekundärantikörper
Klonalität
121

88
Polyklonal
Konjugat
105

14

12

9

6

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
173

87

56

42

33

32

31

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC)

Kreuzreaktivität (Details)

Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Aufreinigung

Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen

Strep-Tag® fusion protein, full-length GFP.

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GFP Reaktivität: Aequorea victoria FACS, IM Wirt: Ziege Polyclonal FITC

Applikationshinweise

WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Kommentare

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer

PBS

Handhabung

Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung

-20 °C

Informationen zur Lagerung

Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Rotmann, Strand, Martiné, Closs: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Synonyme

green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse

Viral Protein