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Neuroligin 1/2/3/4 Antikörper (AA 1-695)

NLGN1/2/3/4 Reaktivität: Maus, Ratte IP, ELISA, WB Wirt: Maus Monoclonal 4F9 unconjugated
Produktnummer ABIN1742285
  • Target
    Neuroligin 1/2/3/4 (NLGN1/2/3/4)
    Bindungsspezifität
    AA 1-695
    Reaktivität
    Maus, Ratte
    Wirt
    Maus
    Klonalität
    Monoklonal
    Applikation
    Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Spezifität
    Recognizes rat and mouse neuroligins 1-3 and rat neuroligin 4.
    Aufreinigung
    purified IgG
    Immunogen
    Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1-695).
    Klon
    4F9
    Isotyp
    IgG2a
  • Applikationshinweise
    WB: 1 : 1000 (AP staining)
    ICC: not recommended
    IHC: not recommended
    Kommentare

    ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handhabung
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

    Fujita-Jimbo, Tanabe, Yu, Kojima, Mori, Li, Iwamoto, Yamagata, Momoi, Momoi: "The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis." in: Molecular autism, Vol. 6, pp. 17, (2015) (PubMed).

    Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

    Bottos, Destro, Rissone, Graziano, Cordara, Assenzio, Cera, Mascia, Bussolino, Arese: "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20782-7, (2010) (PubMed).

    De Jaco, Comoletti, Kovarik, Gaietta, Radic, Lockridge, Ellisman, Taylor: "A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9667-76, (2006) (PubMed).

    Chih, Engelman, Scheiffele: "Control of excitatory and inhibitory synapse formation by neuroligins." in: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, (2005) (PubMed).

  • Target
    Neuroligin 1/2/3/4 (NLGN1/2/3/4)
    Andere Bezeichnung
    Neuroligin
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