pADPR Antikörper (Biotin)

Produktnummer ABIN1741704

Produktdetails

Target: pADPR

Reaktivität: Alle Spezies

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Produktmerkmale: Recognizes DNA-damage and apoptosis related poly-ADP ribosylated proteins and poly (ADP-ribose) polymer

Aufreinigung: Protein A affinity column

Immunogen: poly(ADP-ribose)

Klon: 10H

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: WB 5 µg/mL,
IH 2 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 with 0,05 % sodium azides

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for 1 year from date of shipment when stored at -20 °C or -70 °C. Stable for at least 1 month at 4 °C.

Referenzen

Forst, Karlberg, Herzog, Thorsell, Gross, Feijs, Verheugd, Kursula, Nijmeijer, Kremmer, Kleine, Ladurner, Schüler, Lüscher: "Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).

Kashima, Idogawa, Mita, Shitashige, Yamada, Ogi, Suzuki, Toyota, Ariga, Sasaki, Tokino: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, (2012) (PubMed).

Chang, Coughlin, Mitchison: "Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function." in: Nature cell biology, Vol. 7, Issue 11, pp. 1133-9, (2005) (PubMed).

Antigendetails

Target: pADPR

Hintergrund: Synonyms: poly(ADP-ribose)
Poly(ADP-ribose) or pADPr is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. pADPr becomes attached to nuclear proteins, and to PARP itself (automodification).