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RUNX3 Antikörper (AA 31-130)

RUNX3 Reaktivität: Human WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1714069
  • Target Alle RUNX3 Antikörper anzeigen
    RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
    Bindungsspezifität
    • 16
    • 15
    • 10
    • 9
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-130
    Reaktivität
    • 105
    • 43
    • 32
    • 15
    • 14
    • 13
    • 2
    • 2
    • 1
    Human
    Wirt
    • 93
    • 15
    • 1
    Kaninchen
    Klonalität
    • 97
    • 12
    Polyklonal
    Konjugat
    • 41
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Dieser RUNX3 Antikörper ist unkonjugiert
    Applikation
    • 96
    • 39
    • 36
    • 28
    • 25
    • 23
    • 14
    • 10
    • 9
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Kreuzreaktivität
    Human
    Homologie
    Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Runx3
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029621 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    CaresBio Laboratory
    No.
    #029621
    Datum
    08.03.2014
    Antigen
    Chargennummer
    131016
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    A431 cells
    Negativkontrolle
    HEK293 cells
    Bewertung
    Strong signal was detected in the positive control, and no signal was detected in the negative control.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Runt-Related Transcription Factor 3 (RUNX3)
    • Catalog number: ABIN1714069
    • Lot number: 131016
    Sekundärantikörper
    • Antibody: Donkey Anti-Rabbit secondary antibody IgG (H+L) AlexaFluor 488
    Full Protocol
    • Antibody staining:
    • A431 and Hek-293 cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
    • Cells were incubated with primary antibody diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
    • Stained cells were imaged with an Olympus IX73 microscope.
    • Isotype Control Staining:
    • All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
    • Stained cells were imaged with an Olympus IX73 microscope.
    • Secondary Antibody Only Control Staining:
    • All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with donkey anti rabbit Alexa488 conjugated secondary antibody for 60 min in dark at RT.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
    • Stained cells were imaged with an Olympus IX73 microscope.
    Anmerkungen
    • No discrepancies reported.
    • Runx3 antibody is recommended for IF on A431 cells according to this protocol.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
    Andere Bezeichnung
    Runx3 (RUNX3 Produkte)
    Synonyme
    runxb antikoerper, RUNX3 antikoerper, CBFA3 antikoerper, frrunx3 antikoerper, runx3 antikoerper, aml2 antikoerper, cbfa3 antikoerper, pebp2ac antikoerper, AML2 antikoerper, Cbfa3 antikoerper, Pebp2a3 antikoerper, PEBP2aC antikoerper, runt-related transcription factor 3 antikoerper, runt related transcription factor 3 antikoerper, runt related transcription factor 3 L homeolog antikoerper, runx3 antikoerper, RUNX3 antikoerper, runx3.L antikoerper, Runx3 antikoerper
    Hintergrund

    Synonyms: RUNX 3, RUNX-3, Runt-related transcription factor 3, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, OncogeneAML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.

    Background: RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.

    Gen-ID
    864
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