MUC1 Antikörper (Mucin 1, Cell Surface Associated)

Details for Product anti-MUC1 Antibody No. ABIN1690718
Antigen
  • CA 15-3
  • CD227
  • EMA
  • H23AG
  • KL-6
  • MAM6
  • MCKD1
  • MUC-1
  • MUC-1/SEC
  • MUC-1/X
  • MUC1/ZD
  • PEM
  • PEMT
  • PUM
  • Muc-1
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • MUC1
  • Muc1
Reaktivität
Human
1164
166
71
18
7
6
5
4
4
3
3
2
1
Wirt
Maus
778
413
4
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser MUC1 Antikörper ist unkonjugiert
61
46
40
37
36
35
11
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
10
10
10
10
3
3
3
3
3
2
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA
520
473
455
404
324
303
160
132
61
43
39
38
35
27
14
14
9
7
5
4
2
2
Optionen
'Independent Validation' Siegel
Chargennummer DABC53/1/001/13
Validierte Anwendung Flow Cytometry
Positivkontrolle MCF7 cells
Negativkontrolle C6/36 insect cell line
Bewertung Strong signal was detected in positive control sample, and no signal above isotype control was seen in negative control sample.
Primärantikörper
  • Antigen: Carbohydrate Antigen 15-3 (CA15-3)
  • Catalog number: ABIN1690718
  • Lot number: DABC53/1/001/13
Isotypkontrolle
  • Antibody: mouse IgG1
Sekundärantikörper
  • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
Kontrollen
  • Positive control: MCF-7 breast tumor cell line
  • Negative control: C6/36 insect cell line
  • Isotype control: cells treated with mouse IgG1 instead of the primary antibody to confirm that primary antibody binding is specific.
Protokoll
  • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
  • Detached cells were washed and resuspended in 100 µL 1X PBS containing 0.5% BSA.
  • 1 µg of anti-CA15-3 or mouse IgG1 isotype control was added to the cells and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA
  • Cells were reuspended in 100 µL 1X PBS containing 0.5% BSA
  • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA
  • Cells were resuspended in 0.3 mL PBS/BSA
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Anmerkungen None
Validierungsbilder
Flow Cytometry validation image for anti-Mucin 1, Cell Surface Associated (MUC1) antibody (ABIN1690718) Figure 1: MCF7 cells stained with anti-CA15-3 (blue) or isotype control (red).
Flow Cytometry validation image for anti-Mucin 1, Cell Surface Associated (MUC1) antibody (ABIN1690718) Figure 2: C6/36 cells stained with anti-CA15-3 (blue) or isotype control (red).
Immunogen Native CA15-3 Antigen
Klon 3C101B121B1
Isotyp IgG1
Produktmerkmale Monoclanal Antibody to CA15-3 like antigen.
Reinigung Protein A affinity column
Güteklasse Highly Pure Grade
Plasmids, Primers & others Plasmide, Primers & weitere MUC1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CA15-3 (MUC1 Antibody Abstract)
Gen-ID 4582
Pathways Negative Regulation of intrinsic apoptotic Signaling
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2.45 mg/mL
Buffer 20 mM PBS (pH 7.4+0.2), 0.1 M NaCl and 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repated freezing and thawing
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Short term storage at 2-8°C, Long term storage at -20 °C is recommended.
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