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TCP1 alpha/CCTA Antikörper (C-Term)

TCP1 Reaktivität: Maus ICC, IF, IP, WB Wirt: Ratte Monoclonal 23c unconjugated
Produktnummer ABIN1686650
$445.90
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 3 bis 4 Werktagen
  • Target Alle TCP1 alpha/CCTA (TCP1) Antikörper anzeigen
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Bindungsspezifität
    • 57
    • 18
    • 17
    • 15
    • 15
    • 13
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 171
    • 117
    • 65
    • 24
    • 22
    • 21
    • 20
    • 19
    • 18
    • 17
    • 14
    • 12
    • 11
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Maus
    Wirt
    • 140
    • 39
    • 22
    • 8
    • 1
    Ratte
    Klonalität
    • 141
    • 69
    Monoklonal
    Konjugat
    • 70
    • 18
    • 13
    • 12
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser TCP1 alpha/CCTA Antikörper ist unkonjugiert
    Applikation
    • 118
    • 63
    • 56
    • 53
    • 52
    • 48
    • 46
    • 37
    • 35
    • 23
    • 13
    • 13
    • 2
    • 1
    • 1
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
    Spezifität
    Detects ~60 & ~92 kDa. The addition of an Ala (LDDA COOH) prevents binding in ELISA to immobilized syn peptide. This ab also recog the p102B' COP subunit of Golgi coatomer. Not react with human HSP60.
    Kreuzreaktivität
    Rind (Kuh), Hund, Hamster, Human, Maus, Kaninchen, Ratte, Schaf
    Aufreinigung
    Protein G Purified
    Immunogen
    Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
    Klon
    23c
    Isotyp
    IgG2c
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN1686650 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Target
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Andere Bezeichnung
    TCP1 alpha (TCP1 Produkte)
    Synonyme
    CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, AI528772, CCT, Cct1, Ccta, TRic, Tcp-1, Tp63, c-cpn, p63, TRiC, CCTalpha, BEST:GH05123, CG5374, Dmel\\CG5374, T-cpl, TCP-1alpha, TCPA_DROME, Tcp1, Tcp1-alpha, gh05123, cct-alpha, ccta, tcp1, tcp1-a, tcp1a, tcp1alpha, CHUNP6875, fa13h08, wu:fa13h08, wu:fc95g06, t-complex 1, T-complex protein 1 subunit alpha, t-complex protein 1, Tcp1-like, t-complex 1 S homeolog, TCP1, cct-1, Tcp1, T-cp1, tcp1.S, tcp1
    Hintergrund
    T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.
    Gen-ID
    21454
    NCBI Accession
    NP_038714
    UniProt
    P11983
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