GLB1 Antikörper (Tag) (Biotin)

Produktnummer ABIN1607765

Produktdetails anti-GLB1 Antikörper (Biotin)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Bindungsspezifität: Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against β-Galactosidase from other sources may occur but have not been specifically determined.

Reinheit: Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified beta-Galactosidase [E.coli].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Beta Galactosidase (E.coli)
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.  The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5.000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1.500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.  A 1:5.000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5.000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10.000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher.
ELISA Dilution: 1:10.000 - 1:50.000
Western Blot Dilution: 1:2.000 - 1:10.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: Beta Galactosidase (GLB1 Produkte)

Synonyme: EBP antikoerper, ELNR1 antikoerper, MPS4B antikoerper, DER antikoerper, XR antikoerper, 0610038K04Rik antikoerper, 1810027P18Rik antikoerper, glb antikoerper, CG9092 antikoerper, Dmel\\CG9092 antikoerper, beta-GAL antikoerper, beta-Gal-1 antikoerper, beta-gal antikoerper, gal antikoerper, lacZ-1 antikoerper, AW125515 antikoerper, Bge antikoerper, Bgl antikoerper, Bgl-e antikoerper, Bgl-s antikoerper, Bgl-t antikoerper, Bgs antikoerper, Bgt antikoerper, C130097A14Rik antikoerper, BGAL antikoerper, zgc:110823 antikoerper, ECK0341 antikoerper, JW0335 antikoerper, GLB antikoerper, galactosidase beta 1 antikoerper, dicarbonyl and L-xylulose reductase antikoerper, dicarbonyl L-xylulose reductase antikoerper, beta-D-galactosidase antikoerper, beta-galactosidase antikoerper, beta galactosidase antikoerper, galactosidase, beta 1 antikoerper, beta-galactosidase-like antikoerper, GLB1 antikoerper, DCXR antikoerper, Dcxr antikoerper, lacZ antikoerper, SSP_RS00525 antikoerper, lacH antikoerper, ECL_03691 antikoerper, Gal antikoerper, Glb1 antikoerper, glb1 antikoerper, VV2_1327 antikoerper, LOC100056371 antikoerper

Hintergrund: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations.

Pathways: Glycosaminoglycan Metabolic Process