Nop5p Antikörper

Produktnummer ABIN1580451

Produktdetails

Target: Nop5p

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Aufreinigung: conc. tissue culture supernatant

Sterilität: Sterile filtered

Klon: 34B12

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µL for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/mL.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Antigendetails

Target: Nop5p

Hintergrund: Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).