GAD Antikörper (Glutamate Decarboxylase 1 (Brain, 67kDa)) (AA 50-100)

Details for Product anti-GAD1 Antibody No. ABIN1574123, Anbieter: Anmelden zum Anzeigen
Antigen
  • CPSQ1
  • GAD
  • SCP
  • GAD-67
  • cpsq1
  • gad1
  • gad1-A
  • gad67
  • GAD67
  • GAD1
  • GLUTAMATE DECARBOXYLASE
  • GLUTAMATE DECARBOXYLASE 1
  • MKP11.30
  • MKP11_30
  • glutamate decarboxylase
  • EP10
  • GAD25
  • GAD44
  • Gad-1
  • Gad67
  • glutamate decarboxylase 1
  • glutamate decarboxylase 1 L homeolog
  • glutamate decarboxylase
  • glutamate decarboxylase 1b
  • GAD1
  • gad1.1.L
  • GAD
  • Gad1
  • gad1b
Epitop
AA 50-100
31
17
16
16
15
10
7
6
6
6
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus), Maus, Human
185
104
90
15
14
9
6
5
5
3
2
1
1
1
Wirt
Kaninchen
137
34
27
6
Klonalität
Polyklonal
Konjugat
Dieser GAD Antikörper ist unkonjugiert
8
8
5
5
5
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
171
75
65
38
21
17
13
13
6
4
2
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH-coupled synthetic peptide within AA 50-100 of human GAD67 .
Isotyp IgG
Spezifität Rabbit Anti-GAD67 Polyclonal Antibody detects endogenous levels of human, mouse, and rat GAD67 protein. It is predicted to react with human GAD67 according to sequence homology.Positive Control: mouse brain and rat brain
Kreuzreaktivität (Details) Rabbit Anti-GAD67 Polyclonal Antibody detects endogenous levels of human, mouse, and rat GAD67 protein. It is predicted to react with human GAD67 according to sequence homology.Positive Control: mouse brain and rat brain
Reinigung Immunoaffinity chromatography
Plasmids, Primers & others Plasmide, Primers & weitere GAD products on genomics-online (e.g. as negative or positive controls)
Antigen Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1)
Andere Bezeichnung GAD67 (GAD1 Antibody Abstract)
Hintergrund Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain. There are two isoforms of GAD, GAD65 (GAD2) and GAD67 (GAD1) (65 and 67 kDa, respectively) encoded by two different genes. The two isoforms have distinct intraneuronal distribution in the brain. There is 64% amino acid identity between the two isoforms. The highest degree of diversity appears to be located at the N-terminus, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. GAD65 is primarily found in nerve terminals. GAD67 has been thought to be primarily located in the nerve cell body and has been identified as a major autoantigen in type 1 diabetes.Rabbit Anti-GAD67 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human GAD67 (Swiss Prot: Q99259).
Applikations-hinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLOther Applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 50-100) antibody (ABIN1574123) Western blot analysis of tissue lysates using 1 µg/mL Rabbit Anti-GAD67 Polyclonal An...
Produkt verwendet in: Maekawa, Kobayashi, Odagaki, Makino, Kumanogoh, Nakamura, Morita, Hayashi: "Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD)." in: Neuroscience letters, Vol. 537, pp. 50-4, 2013 (PubMed).

Ketzef, Kahn, Weissberg, Becker, Friedman, Gitler: "Compensatory network alterations upon onset of epilepsy in synapsin triple knock-out mice." in: Neuroscience, Vol. 189, pp. 108-22, 2011 (PubMed).

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