ENO2/NSE Antikörper (N-Term)

Produktnummer ABIN1574103

Produktdetails anti-ENO2/NSE Antikörper

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Spezifität: Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with beta enolase according to sequence homology.

Kreuzreaktivität (Details): Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with beta enolase according to sequence homology.

Aufreinigung: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from N-terminal of human NSE .

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: NSE (ENO2 Produkte)

Synonyme: ENO2 antikoerper, DKFZp459B1817 antikoerper, NSE antikoerper, AI837106 antikoerper, D6Ertd375e antikoerper, Eno-2 antikoerper, RNEN3 antikoerper, eno3 antikoerper, wu:fc09h05 antikoerper, zgc:92418 antikoerper, enolase 2 antikoerper, enolase 2 (gamma, neuronal) antikoerper, enolase 2, gamma neuronal antikoerper, enolase 2, gamma, neuronal antikoerper, ENO2 antikoerper, Eno2 antikoerper, eno2 antikoerper

Hintergrund: Enolase is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits (alpha, beta and gamma) that can form homo and heterodimers. Homodimers of gamma enolase are neuronal-specific. NSE (neuron specific enolase) is expressed primarily in neurons. It is also found in elevated levels in plasma in certain neoplasias including pediatric neuroblastoma and small cell lung cancer. NSE is widely used as a diagnostic marker in a variety of clinical assays.Rabbit Anti-NSE Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human NSE (Swiss Prot: P09104).