Geminin Antikörper (AA 50-100)

Produktnummer ABIN1574066

Produktdetails anti-Geminin Antikörper

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Bindungsspezifität: AA 50-100

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Geminin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Spezifität: Geminin Antibody, pAb, Rabbit detects endogenous levels of human geminin protein.

Aufreinigung: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 50-100 of human geminin protein .

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Geminin Antikörper (ABIN1574066)

Wang, Kirschner: "Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex." in: Nature cell biology, Vol. 15, Issue 7, pp. 797-806, (2013) (PubMed).

Details zu Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Andere Bezeichnung: Geminin (GMNN Produkte)

Synonyme: CG3183 antikoerper, Dm Geminin antikoerper, Dm geminin antikoerper, Dmel\\CG3183 antikoerper, Gem antikoerper, Geminin antikoerper, anon-WO0257455.1 antikoerper, gem antikoerper, l(2)k14019 antikoerper, v(2)k09107 antikoerper, sb:cb254 antikoerper, zgc:66217 antikoerper, GB16871 antikoerper, GMNN antikoerper, geminin antikoerper, dyak_GLEANR_833 antikoerper, DyakGE24654 antikoerper, GE24654 antikoerper, LOC100049517 antikoerper, AW546347 antikoerper, geminin, DNA replication inhibitor antikoerper, CG3183 gene product from transcript CG3183-RA antikoerper, geminin antikoerper, GE24654 gene product from transcript GE24654-RA antikoerper, GMNN antikoerper, geminin antikoerper, gmnn antikoerper, LOC410093 antikoerper, Dyak\geminin antikoerper, Gmnn antikoerper

Hintergrund: Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.Geminin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).

Pathways: EGFR Signaling Pathway, DNA Replication