HAT-Tag Antikörper

Produktnummer ABIN1573907

Produktdetails

Target: HAT-Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Sequenz: KDHLIHNVHK EFHAHAHNK

Spezifität: Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal HAT tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 5 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH7.4, containing 0.02 % sodium azid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen

Chandrasekera, Wan, Gizewski, Auchampach, Lasley: "Adenosine A1 receptors heterodimerize with ?1- and ?2-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling." in: Cellular signalling, Vol. 25, Issue 4, pp. 736-42, (2013) (PubMed).

Okada, Yamaura, Suzuki, Itoh, Osada, Takahashi: "Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 165, Issue 2, pp. 83-9, (2013) (PubMed).

Xiao, Zhou, Sun, Feng, Du, Gao, Ji, Yang, Wang, Han, Langford, Lei: "Apa is a trimeric autotransporter adhesin of Actinobacillus pleuropneumoniae responsible for autoagglutination and host cell adherence." in: Journal of basic microbiology, Vol. 52, Issue 5, pp. 598-607, (2012) (PubMed).

Antigendetails

Target: HAT-Tag

Substanzklasse: Tag

Hintergrund: Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.