E Tag Antikörper

Produktnummer ABIN1573902

Produktdetails anti-E Tag Antikörper

Target: E Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Sequenz: GAPVPYPDPL EPR

Spezifität: This Antibody recognizes C-terminal, N-terminal, and internal E tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: E epitope tag peptide GAPVPYPDPLEPR conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-E Tag Antikörper (ABIN1573902)

Du, Zhu, Wu, Fang, Zhou: "Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus." in: International journal of nanomedicine, Vol. 14, pp. 9337-9349, (2020) (PubMed).

Details zu E Tag

Target: E Tag

Andere Bezeichnung: E-tag (E Tag Produkte)

Substanzklasse: Tag

Hintergrund: Rabbit Anti-E-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.