HA-Tag Antikörper

Details zu Produkt Nr. ABIN1558265, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
416
41
19
7
4
3
2
1
1
Wirt
Ratte
215
187
34
31
21
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
43
30
22
9
9
8
7
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
400
189
126
117
77
75
74
30
15
13
11
11
9
5
4
4
4
4
4
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz YPYDVPDYA
Klon 7C9
Isotyp IgG2a
Spezifität The antibody recognizes an epitope located within the aminoterminal sequence YPYDVPDYA (residues 98-106) derived from the influenza hemagglutinin (HA) protein. The antibody recognizesthe HA tag sequence at either the N- or the C-terminus of the fusion protein.
Sensitivität Sensitivity of rat HA antibody 7C9 goes down to 10 ng of purified Sumo-HA and HA-GFP protein.
Reinigung Purifed antibody
Andere Bezeichnung HA Tag
Substanzklasse Tag
Hintergrund Epitope tags are short peptide sequences of known epitopes, fused to recombinant proteins. Anti-tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. They are useful for specific visualization, immunoprecipitation or purification of recombinant proteins and protein complexes.
Applikations-hinweise Western blot: 1 µg/mL
Immunoprecipitation: recommended starting concentration 4 µg/IP
ELISA: not tested
IF: not tested
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS, 0,09% Sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1558265) HEK293T cell extract expressing mCherry HA-Myc-His. Primary antibody: 1 µg/ml. Second...
 image for anti-HA-Tag antibody (ABIN1558265) Sensitivity of rat HA antibody goes down to 10 ng of purified Sumo-HA and HA-GFP prot...
Produkt verwendet in: Liu, Pedersen, Schultz-Larsen, Aguilar, Madriz-Ordeñana, Hovmøller, Thordal-Christensen: "The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases." in: The New phytologist, 2016 (PubMed).

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