beta Actin Antikörper (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153388
Antigen
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Epitop
N-Term
131
38
17
17
10
7
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Vogel, Chinese Hamster, Fisch, Human, Maus, Schwein, Primate, Kaninchen, Ratte (Rattus)
554
326
281
97
86
74
64
54
51
43
36
33
21
14
14
11
10
10
9
7
6
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Wirt
Kaninchen
355
238
14
7
2
1
Klonalität
Polyklonal
Konjugat
Dieser beta Actin Antikörper ist unkonjugiert
34
31
16
7
7
7
7
7
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Blocking Peptide (BP), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
523
230
158
106
82
68
41
37
34
13
13
12
12
8
2
1
1
1
1
Optionen
Immunogen A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Reinigung Immunogen affinity purified
Plasmids, Primers & others Plasmide, Primers & weitere beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung beta-Actin (ACTB Antibody Abstract)
Hintergrund Gene Symbol: ACTB
Gen-ID 60
UniProt P60709
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Applikations-hinweise Western Blot 1:100-1:2000, Simple Western 1:12.5, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Blocking/Neutralizing, Proximity Ligation Assay, Enzyme ImmunoassayPrior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC. Use in Proximity Ligation Assay reported in scientific literature (PMID 24740405). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.
Bilder des Herstellers
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Rabbit polyclonal to beta actin (ABIN153388) at 1/5000.
Simple Western (SimWes) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Simple Western: beta-Actin Antibody [ABIN153388] - Simple Western lane view shows a s...
Flow Cytometry (FACS) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Flow Cytometry: beta-Actin Antibody [ABIN153388] - analysis of HeLa cells using mouse...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Western Blot: beta-Actin Antibody [ABIN153388] - Western blot analysis of Jurkat, COS...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Immunohistochemistry-Paraffin: beta-Actin Antibody [ABIN153388] - Analysis of Beta Ac...
Immunofluorescence (IF) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [ABIN153388] - IF Confoca...
Flow Cytometry (FACS) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153388) Flow Cytometry: beta-Actin Antibody - An intracellular stain was performed on HeLa c...
Produkt verwendet in: Abdelbaset-Ismail, Cymer, Borkowska-Rzeszotek, Brzeźniakiewicz-Janus, Rameshwar, Kakar, Ratajczak, Ratajczak: "Bioactive Phospholipids Enhance Migration and Adhesion of Human Leukemic Cells by Inhibiting Heme Oxygenase 1 (HO-1) and Inducible Nitric Oxygenase Synthase (iNOS) in a p38 MAPK-Dependent Manner." in: Stem cell reviews, Vol. 15, Issue 1, pp. 139-154, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Poggio, Songia, Moschetta, Valerio, Myasoedova, Perrucci, Pompilio: "MiRNA profiling revealed enhanced susceptibility to oxidative stress of endothelial cells from bicuspid aortic valve." in: Journal of molecular and cellular cardiology, Vol. 131, pp. 146-154, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Zhang, Shi, Gao, Liu, Wang, Tan: "TSPAN1 upregulates MMP2 to promote pancreatic cancer cell migration and invasion via PLCγ." in: Oncology reports, Vol. 41, Issue 4, pp. 2117-2125, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Shah, Shimell, Bamji: "Regulation of dendrite morphology and excitatory synapse formation by zDHHC15." in: Journal of cell science, Vol. 132, Issue 13, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

Schneider, Aras, Williams, Koprich, Brotchie, Singh: "GM1 Ganglioside Modifies α-Synuclein Toxicity and is Neuroprotective in a Rat α-Synuclein Model of Parkinson's Disease." in: Scientific reports, Vol. 9, Issue 1, pp. 8362, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

So, Sun, Wu, Driesman, Leggett, Isaac, Spangler, Dubielecka-Szczerba, Reginato, Liang: "Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development." in: Journal of cellular physiology, 2019 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Guo, Zhang, Bratton, Mottamal, Liu, Ma, Zheng, Zhong, Yang, Wiese, Wu, Ellis, Matossian, Burow, Miele, Houtman, Wang: "ZB716, a steroidal selective estrogen receptor degrader (SERD), is orally efficacious in blocking tumor growth in mouse xenograft models." in: Oncotarget, Vol. 9, Issue 6, pp. 6924-6937, 2018 (PubMed).

Güçlü, Doganlar, Gürlü, Özal, Dogan, Turhan, Doganlar: "Effects of cisplatin-5-fluorouracil combination therapy on oxidative stress, DNA damage, mitochondrial apoptosis, and death receptor signalling in retinal pigment epithelium cells." in: Cutaneous and ocular toxicology, Vol. 37, Issue 3, pp. 291-304, 2018 (PubMed).

Shi, Xue, Wang, Wang, Li, Zhao, Li: "An improved method for increasing the efficiency of gene transfection and transduction." in: International journal of physiology, pathophysiology and pharmacology, Vol. 10, Issue 2, pp. 95-104, 2018 (PubMed).

Tseng, Kim, Li, Cho: "Myeloperoxidase Negatively Regulates Neutrophil-Endothelial Cell Interactions by Impairing αMβ2 Integrin Function in Sterile Inflammation." in: Frontiers in medicine, Vol. 5, pp. 134, 2018 (PubMed).

Tian, Zhang, Piao, Li, Sheng, Zhou, Dong, Zhang, Yan, Shang, Zhao, Xu, Liu, Shi: "Silencing Tspan1 inhibits migration and invasion, and induces the apoptosis of human pancreatic cancer cells." in: Molecular medicine reports, Vol. 18, Issue 3, pp. 3280-3288, 2018 (PubMed).

Ross, Barnett, Faulkner, Hannapel, Parent: "Sucrose ingestion induces glutamate AMPA receptor phosphorylation in dorsal hippocampal neurons: Increased sucrose experience prevents this effect." in: Behavioural brain research, 2018 (PubMed).

Sobolewski, Varma, Adams, Anderson, Schneider, Cory-Slechta: "Developmental Lead Exposure and Prenatal Stress Result in Sex-Specific Reprograming of Adult Stress Physiology and Epigenetic Profiles in Brain." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 163, Issue 2, pp. 478-489, 2018 (PubMed). (Probematerial (Species): Mouse (Murine)).

Molinuevo, Fernández, Cortizo, McCarthy, Schurman, Sedlinsky: "Advanced glycation end products and strontium ranelate promote osteogenic differentiation of vascular smooth muscle cells in vitro: Preventive role of vitamin D." in: Molecular and cellular endocrinology, Vol. 450, pp. 94-104, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Taglialatela, Alvarez, Leuzzi, Sannino, Ranjha, Huang, Madubata, Anand, Levy, Rabadan, Cejka, Costanzo, Ciccia: "Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers." in: Molecular cell, Vol. 68, Issue 2, pp. 414-430.e8, 2017 (PubMed).

Segal, Pancaro, Bonney, Marchand: "Noninfectious Fever in the Near-Term Pregnant Rat Induces Fetal Brain Inflammation: A Model for the Consequences of Epidural-Associated Maternal Fever." in: Anesthesia and analgesia, Vol. 125, Issue 6, pp. 2134-2140, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Kramer, Wu, Crowe: "Tumor suppressor control of the cancer stem cell niche." in: Oncogene, Vol. 35, Issue 32, pp. 4165-78, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Lee, Lee, Bae, Kang, Kim: "Genome-wide identification of target genes for miR-204 and miR-211 identifies their proliferation stimulatory role in breast cancer cells." in: Scientific reports, Vol. 6, pp. 25287, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Savas, Wei, Hsu, Falck, Guengerich, Capdevila, Johnson et al.: "20-Hydroxyeicosatetraenoic Acid (HETE)-dependent Hypertension in Human Cytochrome P450 (CYP) 4A11 Transgenic Mice: NORMALIZATION OF BLOOD PRESSURE BY SODIUM RESTRICTION, HYDROCHLOROTHIAZIDE, OR ..." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16904-19, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)).

Douglas, Gardner, Salapa, Levin et al.: "Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple ..." in: Journal of clinical & cellular immunology, Vol. 7, Issue 2, pp. 402, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

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