CD36 Antikörper (CD36 Molecule (thrombospondin Receptor)) (AA 300-400)

Details for Product anti-CD36 Antibody No. ABIN152912, Anbieter: Anmelden zum Anzeigen
Antigen
  • BDPLT10
  • CHDS7
  • FAT
  • GP3B
  • GP4
  • GPIV
  • PASIV
  • SCARB3
  • Fat
  • Scarb3
  • GPIIIB
  • PAS-4
  • zgc:92513
  • CD36 molecule
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Cd36
  • cd36
Epitop
AA 300-400
16
16
15
11
6
6
4
4
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus
362
151
92
8
7
4
4
3
3
3
1
Wirt
Kaninchen
254
138
48
12
2
Klonalität
Polyklonal
Konjugat
Dieser CD36 Antikörper ist unkonjugiert
53
27
21
20
10
9
8
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
292
143
134
90
65
41
40
39
37
29
13
3
3
3
3
3
3
2
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen A synthetic peptide mapping to a region of human CD36 between residues 300-400. [UniProt# P16671]
Reinigung Immunogen affinity purified
Plasmids, Primers & others Plasmide, Primers & weitere CD36 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CD36 (CD36 Antibody Abstract)
Hintergrund Gene Symbol: CD36
Molekulargewicht Theoretical MW: 75 kDa
Gen-ID 948
UniProt P16671
Pathways TLR Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism
Applikations-hinweise Western Blot 1:2000-1:10000, Immunocytochemistry/ImmunofluorescenceThis CD36 antibody is useful for Western blot, where a band is seen ~75-80 kDa. May see a very faint band ~130 kDa with a flash exposure and several bands with exposure times longer than 3 seconds. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27226602).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for CD36 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1 hour.
. Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.
Bilder des Herstellers
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 300-400) antibody (ABIN152912) anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 300-400) antibody
Produkt verwendet in: Yang, Zhang, Chen, Li, Sun, Liu, Liu, Yu, Li, Han, Duan: "Activation of Peroxisome Proliferator-activated Receptor γ (PPARγ) and CD36 Protein Expression: THE DUAL PATHOPHYSIOLOGICAL ROLES OF PROGESTERONE." in: The Journal of biological chemistry, Vol. 291, Issue 29, pp. 15108-18, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kriska, Cepura, Gauthier, Campbell: "Role of macrophage PPARγ in experimental hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 1, pp. H26-32, 2014 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Wong, Kyle, Croft, Quinn, Jessup, Yeap: "Modulation of macrophage fatty acid content and composition by exposure to dyslipidemic serum in vitro." in: Lipids, Vol. 46, Issue 4, pp. 371-80, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Gieseg, Amit, Yang, Shchepetkina, Katouah: "Oxidant production, oxLDL uptake, and CD36 levels in human monocyte–derived macrophages are downregulated by the macrophage-generated antioxidant 7,8-dihydroneopterin." in: Antioxidants & redox signaling, Vol. 13, Issue 10, pp. 1525-34, 2010 (PubMed).

Truong, Aubin, Falstrault, Brodeur, Brissette: "SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells." in: Cell biochemistry and function, Vol. 28, Issue 6, pp. 480-9, 2010 (PubMed).