ABCG1 Antikörper (ATP-Binding Cassette, Sub-Family G (WHITE), Member 1) (Internal Region)

Details for Product anti-ABCG1 Antibody No. ABIN152900, Anbieter: Anmelden zum Anzeigen
Antigen
  • ABCG1
  • zgc:162197
  • ABC8
  • WHITE1
  • AW413978
  • Abc8
  • White
  • ATP binding cassette subfamily G member 1
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • ABCG1
  • abcg1
  • Abcg1
Epitop
Internal Region, AA 300-400
15
14
10
6
6
6
5
4
4
4
3
2
2
1
1
1
1
Reaktivität
Chinese Hamster, Hamster, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
104
59
47
12
12
11
11
1
1
1
1
1
1
Wirt
Kaninchen
101
6
1
Klonalität
Polyklonal
Konjugat
Dieser ABCG1 Antikörper ist unkonjugiert
7
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Western Blotting (WB)
83
50
30
25
19
14
13
13
6
4
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Reinigung Immunogen affinity purified
Plasmids, Primers & others Plasmide, Primers & weitere ABCG1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung ABCG1 (ABCG1 Antibody Abstract)
Hintergrund Gene Symbol: ABCG1
Gen-ID 9619
UniProt P45844
Pathways Lipid Metabolism
Applikations-hinweise Western Blot 1:500, Immunohistochemistry 1:100-1:400, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:100-1:400, In vitro assayThis ABCG1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) were tested, where a band was observed at ~90 kDa. Preliminary results suggest that this antibody does not appear to work in Immunoprecipitation.In vivo assay was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23185679)
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for ABCG1 Antibody Western Blot Protocol for NB 400-132Protein Extraction:
1. After washing with PBS, cells (mouse peritoneal macrophages grown in a 60 mm dish) in 300 mL of cold lysis buffer [50 mM Tris, pH 7.5, 100 mM NaCl, 1 % Triton x-100, 1 % NaC24H39O4, 50 mM NaF, 1 mM Na3VO4, 1 mM PMSF, 50 µg/ml of aprotinin and 50 µg/ml of leupeptin] are lysed by scraping and sonicating for 25 seconds on ice.
. Spin cellular lysate for 10 min. at 13,000 rpm at 4 degrees Celcius.
. Save supernatant and store at -20 degrees Celcius. Western blotting:
. Determine protein content by Lowry method.
. Load 40 µg of cellular protein [pre-boiled for 5 min. in sample buffer] on a 7.5 % SDS-PAGE separating gel.
. Run electrophoresis for 90 min. at RT in 1x electrophoresis buffer.
. After electrophoresis, equilibrate the gel and nitrocellulose membrane in transfer buffer.
. Transfer proteins in 1x transfer buffer for 1 hour at 100 volts and RT.
. Block the membrane in 10 mL of TBS with 5 % NFDM for 1 hour at RT.
. After a quick rinse with TBS-T (0.5 % Tween-20), membrane is incubated in diluted anti-ABCG1 (cat# NB 400-132) in 1 % NFDM/TBS for 1.5 hours at RT.
. Wash the membrane in 25 mL of TBS-T for 3x 5 minutes at RT.
. Incubate the membrane in 10 mL of diluted secondary antibody (Anti-Rabbit IgG-HRP Conjugate) in 1 % NFDM/TBS for 1 hour at RT.
. Wash the membrane with 25 mL of TBS-T for 3x 5 minutes at RT.
. Incubate the membrane in ECL Western blotting detection reagents for 1 minute.
. Expose to film for ~2 minutes (adjust time as needed for best image).IHC-FFPE sectionsI. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.
Bilder des Herstellers
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1) (AA 300-400), (Internal Region) antibody (ABIN152900) Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using ABIN152900. Lane 1:...
Immunohistochemistry (IHC) image for anti-ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1) (AA 300-400), (Internal Region) antibody (ABIN152900) Staining of lung, alveolar macrophages using ABIN152900.
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1) (AA 300-400), (Internal Region) antibody (ABIN152900) Western Blot: ABCG1 Antibody [ABIN152900] - Detection of ABCG1 in J774 mouse macropha...
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1) (AA 300-400), (Internal Region) antibody (ABIN152900) Western Blot: ABCG1 Antibody [ABIN152900] - Western blot analysis of Raw 264.7 (A), a...
Intracellular Flow Cytometry (ICFC) image for anti-ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1) (AA 300-400), (Internal Region) antibody (ABIN152900) Flow (Intracellular): ABCG1 Antibody - RAW 246.7 cells were either untreated (A) or ...
Produkt verwendet in: Cuffe, Liu, Key, Boudyguina, Sawyer, Weckerle, Bashore, Fried, Chung, Parks: "Targeted Deletion of Adipocyte Abca1 (ATP-Binding Cassette Transporter A1) Impairs Diet-Induced Obesity." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 38, Issue 4, pp. 733-743, 2018 (PubMed).

Li, Shen, Ding, Wang: "Toll-like receptor 2 downregulates the cholesterol efflux by activating the nuclear factor-κB pathway in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 198-204, 2018 (PubMed).

Wang, Eftekhari, Schachner, Ignatova, Palme, Schilcher, Ladurner, Heiss, Stangl, Dirsch, Atanasov: "Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux." in: Scientific reports, Vol. 8, Issue 1, pp. 11061, 2018 (PubMed).

Mariani, Malm, Lamb, Jay, Neilson, Casali, Medarametla, Landreth: "Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease." in: Scientific reports, Vol. 7, pp. 42270, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

de Aguiar Vallim, Lee, Merriott, Goulbourne, Cheng, Cheng, Gonen, Allen, Palladino, Ford, Wang, Baldán, Tarling: "ABCG1 regulates pulmonary surfactant metabolism in mice and men." in: Journal of lipid research, Vol. 58, Issue 5, pp. 941-954, 2017 (PubMed).

Canfrán-Duque, Rotllan, Zhang, Fernández-Fuertes, Ramírez-Hidalgo, Araldi, Daimiel, Busto, Fernández-Hernando, Suárez: "Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis." in: EMBO molecular medicine, Vol. 9, Issue 9, pp. 1244-1262, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Daniil, Zannis, Chroni: "Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux." in: PLoS ONE, Vol. 8, Issue 6, pp. e67993, 2016 (PubMed). Weitere Details: Western Blotting

Leng, Iwanowycz, Saaoud, Wang, Wang, Sergin, Razani, Fan: "Ursolic acid enhances macrophage autophagy and attenuates atherogenesis." in: Journal of lipid research, Vol. 57, Issue 6, pp. 1006-16, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Zhang, Hu, Zhong, Wang, Yang, Liu, Li, Wang, Zhou, Zhang, Xu, Bu, Zhuang: "Quercetin stabilizes apolipoprotein E and reduces brain Aβ levels in amyloid model mice." in: Neuropharmacology, Vol. 108, pp. 179-92, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Tarling, Edwards: "Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both ABCG1-L550 and ABCG1-P550-Brief Report." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 36, Issue 7, pp. 1323-7, 2016 (PubMed). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Mostafa, Hamdy, Abdel-Rahman, El-Mesallamy: "Effect of vildagliptin and pravastatin combination on cholesterol efflux in adipocytes." in: IUBMB life, Vol. 68, Issue 7, pp. 535-43, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Aryal, Rotllan, Araldi, Ramírez, He, Chousterman, Fenn, Wanschel, Madrigal-Matute, Warrier, Martín-Ventura, Swirski, Suárez, Fernández-Hernando: "ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression." in: Nature communications, Vol. 7, pp. 12313, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Lai, Azzam, Lin, Rai, Lowe, Gabor, Madenspacher, Aloor, Parks, Näär, Fessler: "MicroRNA-33 Regulates the Innate Immune Response via ATP Binding Cassette Transporter-mediated Remodeling of Membrane Microdomains." in: The Journal of biological chemistry, Vol. 291, Issue 37, pp. 19651-60, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Cheng, Gaddis, Wu, McSkimming, Haynes, Taylor, McNamara, Sorci-Thomas, Hedrick: "Loss of ABCG1 influences regulatory T cell differentiation and atherosclerosis." in: The Journal of clinical investigation, Vol. 126, Issue 9, pp. 3236-46, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Shi, Mao, Zhong, Liu, Zhao, Yu, Zhu, Wei, Zhu, Sun, Mao, Zeng: "Lanatoside C Promotes Foam Cell Formation and Atherosclerosis." in: Scientific reports, Vol. 6, pp. 20154, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Wang, Palme, Rotter, Schilcher, Cukaj, Wang, Ladurner, Heiss, Stangl, Dirsch, Atanasov: "Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages." in: Molecular nutrition & food research, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (IHC) (Probematerial (Species): Human).

Jiang, Li: "Activation of PPARγ does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI." in: Biochemical and biophysical research communications, Vol. 482, Issue 4, pp. 849-856, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Lee, Lu, Yu, Lee, Tsai: "β Common Receptor Mediates Erythropoietin-Conferred Protection on OxLDL-Induced Lipid Accumulation and Inflammation in Macrophages." in: Mediators of inflammation, Vol. 2015, pp. 439759, 2016 (PubMed).

Park, Paek, Shin, Lee, Lim, Kang: "Purple perilla extracts with α-asarone enhance cholesterol efflux from oxidized LDL-exposed macrophages." in: International journal of molecular medicine, Vol. 35, Issue 4, pp. 957-65, 2015 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Kuo, Leon, Chung, Huang, Sontag, Reardon, Getz, Tirrell, Fang: "Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles." in: Journal of materials chemistry. B, Materials for biology and medicine, Vol. 2, Issue 46, pp. 8142-8153, 2015 (PubMed).

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