ABCA1 Antikörper (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300)

Details for Product anti-ABCA1 Antibody No. ABIN152883, Anbieter: Anmelden zum Anzeigen
Antigen
  • ABC-1
  • ABC1
  • CERP
  • HDLDT1
  • TGD
  • ABCA1
  • Abc1
  • ATP-binding cassette A1
  • Arabidopsis thaliana ATP-binding cassette A1
  • AtABCA1
  • ATP binding cassette subfamily A member 1
  • ATP-binding cassette, sub-family A (ABC1), member 1
  • ATP-binding cassette A1
  • ABCA1
  • Abca1
  • abca1
Epitop
AA 1100-1300
25
21
16
15
14
12
8
6
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reaktivität
Huhn, Hund, Hamster, Human, Maus, Marder, Schwein, Ratte (Rattus)
154
137
84
21
16
14
14
13
12
3
2
2
1
1
Wirt
Kaninchen
105
54
22
4
Klonalität
Polyklonal
Konjugat
Dieser ABCA1 Antikörper ist unkonjugiert
13
8
8
8
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Applikation
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
105
72
41
36
34
33
32
26
12
12
4
3
2
2
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Reinigung Immunogen affinity purified
Andere Bezeichnung ABCA1 (ABCA1 Antibody Abstract)
Hintergrund Gene Symbol: ABCA1
Molekulargewicht Theoretical MW: 220 kDa
Gen-ID 19
UniProt O95477
Pathways Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
Applikations-hinweise Western Blot 1:500, Simple Western 1:50, Chromatin Immunoprecipitation, Flow Cytometry 1:400, ELISA, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen, Gel Supershift AssayThis ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716, 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). In Western blot a band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation and ELISA were also reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Charge. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for ABCA1 Antibody Western BlotThe experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1 expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of NB 400-105 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.NOTE: An important factor in detecting ABCA1 is in the cell type used. ABCA1 is expressed in very low levels in most cell types. Therefore, ABCA1 expression needs to be induced by using 22-hydroxycholesterol and 9-cis-retinoic acid as ligands for the transcription factor LXR.
1. Without heating at all (leave at room temp for about 15 to 20 minutes with Beta-mercaptoethanol), load 40 µg post-nuclear lysates* to 7.5 % or 4-15 % Tris-HCL SDS gel (Bio-RAD) in sample buffer. Do NOT boil the samples. (NP-40 will not interfere with the running of the protein on SDS-PAGE.)
. Transfer to nitrocellulose membrane at 100V 1hr or 30V overnight.
. Block membrane in 5 % milk in TBS-T for at least 1 hr. Wash with TBS-T 5 minutes.
. Blot with anti-ABCA1 antibody in 3 % milk in TBS-T for 1 hour.
. Wash with TBS-T 3 times, 10 minutes each.
. Blot with donkey anti-rabbit secondary, antibody (Amersham) at 1:2000 in 3 % milk in TBS-T for 1 hour.
. Wash with TBS-T 3 times, 10 minutes each.
. Detect with chemiluminescent reagent (Pierce).
. Expose to X-ray film, 30 sec, 1 min, and 3 min. TBS-T: Tris-buffered-saline with Tween-20See also the specific references mentioned in the datasheet. *Post-nuclear lysate is the result of sonication or dounce homogenization of lysate, centrifugation at low-speed, and the removal of nuclei. The resulting supernatant is called post-nuclear and contains cytosolic and membrane proteins without any of the nuclear components.IHC-FFPE sectionsI. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: -Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. -Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.
Bilder des Herstellers
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Western Blot analysis of ABCA1, using ABIN152883. Samples: 40 ug of total cell post-n...
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Detection of ABCA1 in mouse peritoneal macrophages using ABIN152883 (Lot L). ECL expo...
Immunohistochemistry (IHC) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (Image 3)
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Western Blot: ABCA1 Antibody [ABIN189744] - Detection of ABCA1 in mouse peritoneal ma...
Flow Cytometry (FACS) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Flow Cytometry: ABCA1 Antibody [ABIN189744] - ABCA-1 FITC conjugated antibody of huma...
Flow Cytometry (FACS) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Flow Cytometry: ABCA1 Antibody [ABIN189744] - ABCA1 antibody was tested at 1: 400 in ...
Immunofluorescence (IF) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [ABIN189744] - ABCA1 antibody ...
Immunofluorescence (IF) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Immunocytochemistry/Immunofluorescence: ABCA1 Antibody - Untreated HepG2 cells were ...
Immunofluorescence (IF) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Immunocytochemistry/Immunofluorescence: ABCA1 Antibody - HepG2 cells were grown to 6...
Flow Cytometry (FACS) image for anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (ABIN152883) Flow (Cell Surface): ABCA1 Antibody - RAW 246.7 cells were either untreated (A) or s...
Produkt verwendet in: Hafiane, Genest: "ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis." in: Atherosclerosis, Vol. 257, pp. 90-99, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Dong, Pan, Zhang, Zhang, Wang, Jiang, Chen, Duan, Wu, Xu, Zhang, Zhu: "Hawthorn (Crataegus pinnatifida Bunge) leave flavonoids attenuate atherosclerosis development in apoE knock-out mice." in: Journal of ethnopharmacology, Vol. 198, pp. 479-488, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Mariani, Malm, Lamb, Jay, Neilson, Casali, Medarametla, Landreth: "Neuronally-directed effects of RXR activation in a mouse model of Alzheimer's disease." in: Scientific reports, Vol. 7, pp. 42270, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Cook, Nelson, Sorrentino, Koenis, Moeton, Scheij, Ottenhoff, Bleijlevens, Loregger, Zelcer: "Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172721, 2017 (PubMed).

Pulakazhi Venu, Adijiang, Seibert, Chen, Shi, Batulan, OBrien: "Heat shock protein 27-derived atheroprotection involves reverse cholesterol transport that is dependent on GM-CSF to maintain ABCA1 and ABCG1 expression in ApoE(-/-) mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 6, pp. 2364-2379, 2017 (PubMed).

Mailleux, Vanmierlo, Bogie, Wouters, Lütjohann, Hendriks, van Horssen: "Active liver X receptor signaling in phagocytes in multiple sclerosis lesions." in: Multiple sclerosis (Houndmills, Basingstoke, England), pp. 1352458517696595, 2017 (PubMed).

Li, Xiu, Wang, Yu, Chi, Qin, Fu, Matsuura, Liu: "The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPARγ." in: Life sciences, Vol. 177, pp. 27-40, 2017 (PubMed).

Morizawa, Hirayama, Ohno, Shibata, Shigetomi, Sui, Nabekura, Sato, Okajima, Takebayashi, Okano, Koizumi: "Reactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway." in: Nature communications, Vol. 8, Issue 1, pp. 28, 2017 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Mouse (Murine)).

Storti, Raphael, Griesser, Klee, Drawnel, Willburger, Scholz, Langmann, von Eckardstein, Fingerle, Grimm, Maugeais: "Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells." in: Experimental eye research, Vol. 165, pp. 65-77, 2017 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Human).

Doran, Ozcan, Cai, Zheng, Fredman, Rymond, Dorweiler, Sluimer, Hsieh, Kuriakose, Tall, Tabas: "CAMKIIγ suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis." in: The Journal of clinical investigation, Vol. 127, Issue 11, pp. 4075-4089, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Widenmaier, Snyder, Nguyen, Arduini, Lee, Arruda, Saksi, Bartelt, Hotamisligil: "NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis." in: Cell, Vol. 171, Issue 5, pp. 1094-1109.e15, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Sallam, Ito, Rong, Kim, van Stijn, Chamberlain, Jung, Chao, Jones, Gilliland, Wu, Su, Tangirala, Tontonoz, Hong: "The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1120-30, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Merkl, Ertl, Handschuh, Aurich, Schäfer-Somi: "The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues." in: Theriogenology, Vol. 85, Issue 6, pp. 1080-9, 2016 (PubMed).

Fournier, Tardivel, Benoist, Vedie, Rousseau-Ralliard, Nowak, Allaoui, Paul: "Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages." in: Biochimica et biophysica acta, Vol. 1861, Issue 4, pp. 331-41, 2016 (PubMed). Weitere Details: Western Blotting

Schäfer-Somi, Palme: "Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability." in: Reproduction in domestic animals = Zuchthygiene, Vol. 51, Issue 2, pp. 232-9, 2016 (PubMed). (Probematerial (Species): Dog (Canine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Shrestha, Hussein, Savas, Ouimet, Barrett, Leone, Yates, Moore, Fisher, Garabedian: "Poly(ADP-ribose) Polymerase 1 Represses Liver X Receptor-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages." in: The Journal of biological chemistry, Vol. 291, Issue 21, pp. 11172-84, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Ollikainen, Tulamo, Lehti, Lee-Rueckert, Hernesniemi, Niemelä, Ylä-Herttuala, Kovanen, Frösen: "Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 7, pp. 689-99, 2016 (PubMed). Weitere Details: Immunocytochemistry,Immunofluorescence

Kemmerer, Wittig, Richter, Brüne, Namgaladze: "AMPK activates LXRα and ABCA1 expression in human macrophages." in: The international journal of biochemistry & cell biology, Vol. 78, pp. 1-9, 2016 (PubMed). Weitere Details: Western Blotting

Hsieh, Koseki, Molusky, Yakushiji, Ichi, Westerterp, Iqbal, Chan, Abramowicz, Tascau, Takiguchi, Yamashita, Welch, Di Paolo, Hussain, Lefkowitch, Rader, Tall: "TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis." in: Nature, Vol. 535, Issue 7611, pp. 303-7, 2016 (PubMed).

Hnatiuk, Ong, Olea, Locatelli, Riegler, Lee, Jen, De Lorenzi, Giménez, Laguens, Wu, Crottogini: "Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-α (HIF1-α) in an Ovine Model of Acute Myocardial Infarction." in: Journal of the American Heart Association, Vol. 5, Issue 7, 2016 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

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