ATM Antikörper (Ataxia Telangiectasia Mutated) (C-Term)

Details for Product anti-ATM Antibody No. ABIN151030, Anbieter: Anmelden zum Anzeigen
Antigen
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ATM serine/threonine kinase L homeolog
  • ATM serine/threonine kinase
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • serine/threonine-protein kinase ATM
  • tefu
  • atm.L
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • LOC5565620
  • MGYG_07634
  • PGTG_14279
  • Atm
Epitop
C-Term
88
39
17
16
16
15
8
8
6
6
6
6
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
356
155
78
27
8
1
1
1
1
Wirt
Kaninchen
230
99
17
9
3
Klonalität
Polyklonal
Konjugat
Dieser ATM Antikörper ist unkonjugiert
12
11
10
9
8
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
218
124
108
87
84
71
58
44
27
21
13
6
4
4
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]
Reinigung Immunogen affinity purified
Andere Bezeichnung ATM (ATM Antibody Abstract)
Hintergrund Gene Symbol: ATM
Molekulargewicht Theoretical MW: 350 kDa
Gen-ID 472
UniProt Q13315
Forschungsgebiet Cancer, DNA/RNA
Pathways p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Applikationshinweise Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2.5 mg/mL
Buffer PBS and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate ...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemical staining using ABIN151030.
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in Raji whole cell lysate.
Flow Cytometry (FACS) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Flow Cytometry: ATM Antibody [ABIN151030] - Analysis of PE conjugate of ABIN151030. A...
Immunohistochemistry (IHC) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemistry: ATM Antibody [ABIN151030] - Staining of Testis, Seminiferous Tu...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunocytochemistry/Immunofluorescence: ATM Antibody [ABIN151030] - HeLa cells were f...
Produkt verwendet in: Li, Li, Kong, Yan, Ahmad, Liu: "Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response." in: Cancer research, Vol. 77, Issue 12, pp. 3169-3180, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." in: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." in: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." in: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014 (PubMed).

Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (PubMed). (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." in: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." in: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." in: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." in: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." in: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." in: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." in: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Hu, Du, Nagabayashi, Seeger, Gatti: "ATM is down-regulated by N-Myc-regulated microRNA-421." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1506-11, 2010 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting,ELISA

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