Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

COL2A1 Antikörper (Chain alpha 1)

COL2A1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schaf WB, IP, IHC (p), IHC (fro), EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1450180
  • Target Alle COL2A1 Antikörper anzeigen
    COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
    Bindungsspezifität
    • 15
    • 14
    • 10
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Chain alpha 1
    Reaktivität
    • 66
    • 32
    • 30
    • 19
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Schaf
    Wirt
    • 70
    • 10
    • 2
    • 1
    Kaninchen
    Klonalität
    • 74
    • 9
    Polyklonal
    Konjugat
    • 50
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser COL2A1 Antikörper ist unkonjugiert
    Applikation
    • 64
    • 37
    • 22
    • 18
    • 17
    • 14
    • 13
    • 13
    • 9
    • 9
    • 8
    • 7
    • 3
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Spezifität
    This product has been prepared by Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Crossreactivity < 1 % against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Aufreinigung
    Immunoaffinity Chromatography.
    Immunogen
    Collagen type II purified from Human knee and Bovine nasal cartilage.
    Top Product
    Discover our top product COL2A1 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
    Abstract
    COL2A1 Produkte
    Synonyme
    ANFH antikoerper, AOM antikoerper, COL11A3 antikoerper, SEDC antikoerper, Col2 antikoerper, Col2a antikoerper, Col2a-1 antikoerper, Del1 antikoerper, Dmm antikoerper, Lpk antikoerper, M100856 antikoerper, Rgsc856 antikoerper, CG2A1A antikoerper, COLLII antikoerper, col2a1a antikoerper, CB11 antikoerper, col2a1 antikoerper, coll2a1 antikoerper, fb38c06 antikoerper, fc10c01 antikoerper, wu:fb38c06 antikoerper, wu:fc10c01 antikoerper, collagen type II alpha 1 chain antikoerper, collagen, type II, alpha 1 antikoerper, collagen, type II, alpha 1 L homeolog antikoerper, collagen, type II, alpha 1 S homeolog antikoerper, collagen, type II, alpha 1a antikoerper, COL2A1 antikoerper, Col2a1 antikoerper, col2a1 antikoerper, col2a1.L antikoerper, col2a1.S antikoerper, col2a1a antikoerper
    Hintergrund
    Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1
    Gen-ID
    1280
    NCBI Accession
    NP_001835
    UniProt
    P02458
    Pathways
    Sensory Perception of Sound, Growth Factor Binding
Sie sind hier:
Kundenservice