VCP Antikörper (FITC)

Produktnummer ABIN1302844

Produktdetails anti-VCP Antikörper (FITC)

Target: VCP (Valosin Containing Protein (VCP))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VCP Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.

Kreuzreaktivität (Details): Mouse, Human

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Klon: Hs-14

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

Kommentare:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-VCP Antikörper (FITC) (ABIN1302844)

Peknicova, Chladek, Hozak: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).

Chládek, P?knicová, Capková, Geussová, Teplá, Madar: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).

Details zu VCP

Target: VCP (Valosin Containing Protein (VCP))

Andere Bezeichnung: VCP (VCP Produkte)

Synonyme: ALS14 antikoerper, IBMPFD antikoerper, TERA antikoerper, p97 antikoerper, CDC48 antikoerper, wu:fd16d05 antikoerper, wu:fj63d11 antikoerper, p93 antikoerper, 3110001E05 antikoerper, p97/VCP antikoerper, 22.26 antikoerper, 7.12 antikoerper, Apple antikoerper, BcDNA.GM02885 antikoerper, BcDNA:GM02885 antikoerper, CG2331 antikoerper, DmTER94 antikoerper, Dmel\CG2331 antikoerper, I antikoerper, Ter94 antikoerper, Tera antikoerper, VCP antikoerper, VCP ATPase antikoerper, VCP/p97 antikoerper, Vcp antikoerper, anon-WO2004063362.65 antikoerper, anon-WO2004063362.67 antikoerper, cdc48 antikoerper, dTERA antikoerper, dVCP antikoerper, dmTERA antikoerper, dvcp antikoerper, l(2)03775 antikoerper, l(2)46CFf antikoerper, l(2)46CFs antikoerper, l(2)46Ch antikoerper, pTER94 antikoerper, ter94 antikoerper, vcp antikoerper, FANCG antikoerper, valosin containing protein antikoerper, valosin containing protein S homeolog antikoerper, valosin-containing protein antikoerper, CG2331 gene product from transcript CG2331-RA antikoerper, zgc:136908 antikoerper, transitional endoplasmic reticulum ATPase antikoerper, VCP antikoerper, vcp.S antikoerper, vcp antikoerper, Vcp antikoerper, Bm1_56585 antikoerper, LOC100136246 antikoerper, TER94 antikoerper, zgc:136908 antikoerper, LOC101109680 antikoerper

Hintergrund: Valosin containing protein,VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,TERA, CDC48

Gen-ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway