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GFP Antikörper (AA 246) (HRP)

GFP Reaktivität: Aequorea victoria WB, ELISA Wirt: Maus Monoclonal 9F9-F9 HRP
Produktnummer ABIN129566
  • Target Alle GFP Antikörper anzeigen
    GFP (Green Fluorescent Protein (GFP))
    Bindungsspezifität
    • 15
    • 15
    • 10
    • 9
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 246
    Reaktivität
    • 179
    • 19
    • 15
    • 15
    • 12
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Aequorea victoria
    Wirt
    • 76
    • 73
    • 35
    • 11
    • 9
    • 6
    • 5
    • 1
    Maus
    Klonalität
    • 123
    • 88
    Monoklonal
    Konjugat
    • 106
    • 14
    • 12
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GFP Antikörper ist konjugiert mit HRP
    Applikation
    • 172
    • 86
    • 56
    • 42
    • 34
    • 33
    • 32
    • 21
    • 16
    • 14
    • 14
    • 14
    • 12
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Kreuzreaktivität
    recombinant GFP (rGFP), enhanced GFP (eGFP)
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
    Immunogentype:Recombinant
    Klon
    9F9-F9
    Isotyp
    IgG
    Top Product
    Discover our top product GFP Primärantikörper
  • Applikationshinweise
    Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with deionized water (or equivalent)
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    GFP (Green Fluorescent Protein (GFP))
    Andere Bezeichnung
    GFP (GFP Produkte)
    Synonyme
    green fluorescent protein antikoerper, gfp antikoerper
    Substanzklasse
    Viral Protein
    Hintergrund
    Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
    Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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