Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
The mabs represent marker for nucleolar organizer region (NOR), putatively staining the protein treacle (described as TCOF1, cf. Gonzales et al.). Mr 240 kDa band in SDS gels and lower molecular weight bands (e.g. 160 kDa band, Gonzales et al.).
Nuclear pore complex-lamina fraction of XLKE-A6 cells (cultured cell line derived from Xenopus laevis).
Immunofluorescence microscopy. Immunoblotting. Immunohistochemistry: Ready to use, 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.