Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Cylicin I (MW 74,788, deduced from sequence analysis), a basic protein (IEP 10.55) of the sperm head cytoskeleton with Mr 74,000 on SDS-PAGE, multiple band proteins with MW 58, 63, 69 and 74 kDa of calyx-associated polypeptides of bovine spermatozoa. Especially useful in discrimination of morphologically altered "round-headed" spermatozoa.
Alexa Fluor 488
Immunohistochemistry on frozen sections: 1/100, 1h at RT: Western blot: 1/2000, ECL method. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
LOC100283036, 4930401M13Rik, CYCL1, cylicin-1, cylicin, basic protein of sperm head cytoskeleton 1, cylicin 1, EDI_239030, LOC100283036, Cylc1, CYLC1
Cylicin 1 has a possible architectural role during spermatogenesis. May be involved in spermatid differentiation. Specific to late spermatogenesis.Synonyms: CYL, CYL1, CYLC1, Cylicin I, Multiple-band polypeptide I