Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
C-FTTSGGHSLG AGLDDSGFSA TSNRGL
The antiserum stains specifically the companion layer (innermost layer of the outer root sheath) of the hair follicle. Although there is a considerable homology of keratin K75 (K6hf) to keratins K5 and K6, no cross-reactivity was observed, neither in Western blotting nor in Immunofluorescence microscopy.
Species reactivity (tested):Human, Mouse.
KLH coupled Synthetic peptide of Human type II keratin K75 (formerly also designated K6hf)
Immunoblotting. Immunohistochemistry on Frozen Sections. Immunofluorescence Microscopy. Working Dilutions: 1/100-1/200 for Immunofluorescence Microscopy (Acetone fixation),1/100 (4 % Paraformaldehyde Fixation) and 1/2,000 for Western Blotting (ECL method). Incubation Time: 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
KRT75, K6HF, KB18, PFB, 4732468K03Rik, AA589387, K6hf, Krt2-6hf, Krtcap1, Kb18, keratin 75, keratin, type II cytoskeletal 75, keratin, type II cytoskeletal 6A, KRT75, LOC466991, LOC100566287, Krt75
KRT75 plays an essential role in hair and nail formation and is an essential component of keratin intermediate filaments in the companion layer of the hair follicle. Variations in this gene have been associated with the hair disorders pseudofolliculitis barbae (PFB) and loose anagen hair syndrome (LAHS).Synonyms: CK75, Cytokeratin-75, K6HF, K75, KB18, KRT75, Keratin, Keratin-6 hair follicle, Keratin-75, Type II keratin-K6hf, type II cytoskeletal 75