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FeSOD Antikörper

FeSOD Reaktivität: Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Mais (Zea mays) WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2749232
  • Target
    FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
    Reaktivität
    Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Mais (Zea mays)
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    No cross-reactivity with: cyanobacteria
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa
    Aufreinigung
    serum
    Immunogen
    overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
  • Applikationshinweise
    Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).
    Kommentare

    The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water.
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Page, Allen, Kropat, Urzica, Karpowicz, Hsieh, Loo, Merchant: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).

    Abdel-Ghany, Müller-Moulé, Niyogi, Pilon, Shikanai: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, (2005) (PubMed).

  • Target
    FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
    Hintergrund
    AGI Code: At4g25100
    Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
    Molekulargewicht
    expected: 25 kDa, apparent: 22 kDa
    UniProt
    A8IGH1
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