CDC20 Antikörper (C-Term)
-
- Target Alle CDC20 Antikörper anzeigen
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
-
Bindungsspezifität
- AA 486-499, C-Term
-
Reaktivität
- Human
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser CDC20 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Sequenz
- Human CDC20 (fizzy) (499 aa 54.7 kDa): 1 maqfafesdl hsllqldapi pnapparwqr kakeaagpap spmraanrsh sagrtpgrtp 61 gkssskvqtt pskpggdryi phrsaaqmev asfllskenq sensqtptkk ehqkawalnl 21 ngfdveeaki lrlsgkpqna pegyqnrlkv lysqkatpgs srktcryips lpdrildape 181 irndyylnlv dwssgnvlav aldnsvylws assgdilqll qmeqpgeyis svawikegny 241 lavgtssaev qlwdvqqqkr lrnmtshsar vgslswnsyi lssgsrsghi hhhdvrvaeh 301 hvatlsghsq evcglrwapd grhlasggnd nlvnvwpsap geggwvplqt ftqhqgavka 361 vawcpwqsnv latgggtsdr hiriwnvcsg aclsavdahs qvcsilwsph ykelisghgf 421 aqnqlviwky ptmakvaelk ghtsrvlslt mspdgatvas aaadetlrlw rcfeldparr 481 rerekasaak sslihqgir
- Spezifität
- This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
- Aufreinigung
- Delipidation and defibrination.
- Immunogen
- Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
- Top Product
- Discover our top product CDC20 Primärantikörper
-
-
- Applikationshinweise
- Western blot: This antibody reacts with human CDC20 (fizzy) (1: 500 - 1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa bandcorresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressingCDC20 can be used as a positive control.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 85 mg/mL (by Refractometry)
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
-
- Target
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Andere Bezeichnung
- CDC20 (CDC20 Produkte)
- Synonyme
- X-FZY antikoerper, ba276h19.3 antikoerper, cdc20a antikoerper, fizzy1 antikoerper, p55cdc antikoerper, CDC20A antikoerper, bA276H19.3 antikoerper, p55CDC antikoerper, 2310042N09Rik antikoerper, C87100 antikoerper, cell division cycle 20 antikoerper, cell division cycle 20 L homeolog antikoerper, cell division cycle 20 homolog (S. cerevisiae) antikoerper, cdc20 antikoerper, CDC20 antikoerper, cdc20.L antikoerper, Cdc20 antikoerper
- Hintergrund
- CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.Synonyms: Cell division cycle protein 20 homolog, p55CDC
- Gen-ID
- 991
- NCBI Accession
- NP_001246
- UniProt
- Q12834
-