CCR5 Antikörper

Produktnummer ABIN1177340

Produktdetails anti-CCR5 Antikörper

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCR5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Klon: 2D7-CCR5

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Immunophenotyping studies of chemokine receptors are recommended to be performed on freshly collected whole blood (< 24 hrs). Incubation with the antibody should be done at room temperature and in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining have been reported to cause a decrease in staining intensity and inconsistent results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CCR5 Antikörper (ABIN1177340)

Hancock: "Chemokines and the pathogenesis of T cell-dependent immune responses." in: The American journal of pathology, Vol. 148, Issue 3, pp. 681-4, (1997) (PubMed).

Wu, Paxton, Kassam, Ruffing, Rottman, Sullivan, Choe, Sodroski, Newman, Koup, Mackay: "CCR5 levels and expression pattern correlate with infectability by macrophage-tropic HIV-1, in vitro." in: The Journal of experimental medicine, Vol. 185, Issue 9, pp. 1681-91, (1997) (PubMed).

Choe, Farzan, Sun, Sullivan, Rollins, Ponath, Wu, Mackay, LaRosa, Newman, Gerard, Gerard, Sodroski: "The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates." in: Cell, Vol. 85, Issue 7, pp. 1135-48, (1996) (PubMed).

Deng, Liu, Ellmeier, Choe, Unutmaz, Burkhart, Di Marzio, Marmon, Sutton, Hill, Davis, Peiper, Schall, Littman, Landau: "Identification of a major co-receptor for primary isolates of HIV-1." in: Nature, Vol. 381, Issue 6584, pp. 661-6, (1996) (PubMed).

Doranz, Rucker, Yi, Smyth, Samson, Peiper, Parmentier, Collman, Doms: "A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors." in: Cell, Vol. 85, Issue 7, pp. 1149-58, (1996) (PubMed).

Dragic, Litwin, Allaway, Martin, Huang, Nagashima, Cayanan, Maddon, Koup, Moore, Paxton: "HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5." in: Nature, Vol. 381, Issue 6584, pp. 667-73, (1996) (PubMed).

Raport, Gosling, Schweickart, Gray, Charo: "Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 17161-6, (1996) (PubMed).

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CD195 (CCR5 Produkte)

Synonyme: CC-CKR-5 antikoerper, CCCKR5 antikoerper, CCR-5 antikoerper, CD195 antikoerper, CKR-5 antikoerper, CKR5 antikoerper, CMKBR5 antikoerper, IDDM22 antikoerper, Ckr5 antikoerper, Cmkbr5 antikoerper, CCR1-L antikoerper, GPCR antikoerper, CCR5 antikoerper, AM4-7 antikoerper, C-C motif chemokine receptor 5 (gene/pseudogene) antikoerper, chemokine (C-C motif) receptor 5 antikoerper, C-C motif chemokine receptor 5 antikoerper, CCR5 antikoerper, Ccr5 antikoerper

Hintergrund: Reacts with the chemokine receptor, CCR5, a seven transmembrane-spanning G protein-associated molecule. CCR5 belongs to the beta chemokine receptor family. It is expressed on a subset of T lymphocytes (CD3+, CD45RO+, CD95+). CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1) alpha and beta. Additionally, CCR5 has been found to be a co-receptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission. Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection. This antibody has been shown to block ligand and gp120 binding. It is also able to neutralize HIV infection.
Synonyms: CCR5

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size