Phospholipase C gamma 1 Antikörper (pTyr783, Tyr783) (Alexa Fluor 647)

Produktnummer ABIN1177236

Produktdetails anti-Phospholipase C gamma 1 Antikörper (Alexa Fluor 647)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Bindungsspezifität: pTyr783, Tyr783

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phospholipase C gamma 1 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated peptide including the tyrosine 783 of human PLCgamma1

Klon: 27-PLC

Isotyp: IgG1

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-Phospholipase C gamma 1 Antikörper (Alexa Fluor 647) (ABIN1177236)

Yu, Fukami, Itoh, Takenawa: "Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton." in: Experimental cell research, Vol. 243, Issue 1, pp. 113-22, (1998) (PubMed).

Details zu Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Andere Bezeichnung: PLC-gamma 1 (PLCG1 Produkte)

Synonyme: NCKAP3 antikoerper, PLC-II antikoerper, PLC1 antikoerper, PLC148 antikoerper, PLCgamma1 antikoerper, plc1 antikoerper, nckap3 antikoerper, plc-ii antikoerper, plc148 antikoerper, plcgamma1 antikoerper, PPLCA antikoerper, AI894140 antikoerper, Cded antikoerper, Plc-1 antikoerper, Plc-gamma1 antikoerper, Plcg-1 antikoerper, fj46f04 antikoerper, wu:fj46f04 antikoerper, wu:fj57a05 antikoerper, phospholipase C gamma 1 antikoerper, phospholipase C gamma 1 L homeolog antikoerper, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 antikoerper, phospholipase C, gamma 1 antikoerper, PLCG1 antikoerper, plcg1.L antikoerper, LOC100633163 antikoerper, Plcg1 antikoerper, plcg1 antikoerper

Hintergrund: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCg1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCg activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLCg1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612464) has been shown to react with human, mouse, and rat cell lysates by western blot.

Pathways: RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling