CrkL Antikörper (pTyr207) (Alexa Fluor 647)

Produktnummer ABIN1177227

Produktdetails anti-CrkL Antikörper (Alexa Fluor 647)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Bindungsspezifität: pTyr207

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CrkL Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human, Mouse, and Rat CrkL Peptide

Klon: K30-391-50-80

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody conjugate is suitable for intracellular staining of human whole blood, peripheral blood mononuclear cells (PBMC), and bone marrow (using BD Phosflow Lyse/Fix Buffer and Perm Buffer III or IV) and cell lines (using BD Cytofix™ Fixation Buffer and BD Phosflow Perm Buffer III or IV).

Kommentare:

BALB/c

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CrkL Antikörper (Alexa Fluor 647) (ABIN1177227)

Feller: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, (2001) (PubMed).

Arai, Nosaka, Kohsaka, Miyasaka, Miura: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." in: Blood, Vol. 93, Issue 11, pp. 3713-22, (1999) (PubMed).

Senechal, Heaney, Druker, Sawyers: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." in: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, (1998) (PubMed).

Details zu CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Andere Bezeichnung: CrkL (CRKL Produkte)

Synonyme: CRKL antikoerper, wu:fa55g05 antikoerper, wu:fj60e01 antikoerper, zgc:63469 antikoerper, 1110025F07Rik antikoerper, AA589403 antikoerper, AI325100 antikoerper, Crkol antikoerper, CRK like proto-oncogene, adaptor protein antikoerper, v-crk avian sarcoma virus CT10 oncogene homolog-like antikoerper, CRKL antikoerper, crkl antikoerper, Crkl antikoerper

Hintergrund: Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in hematopoietic cells that express the BCR-ABL fusion protein. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.50.80 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.

Pathways: Interferon-gamma Pathway, Neurotrophin Signalübertragung, Signaling of Hepatocyte Growth Factor Receptor