SRC Antikörper (V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian)) (pTyr418) (Alexa Fluor 488)

Details for Product anti-SRC Antibody No. ABIN1177181, Anbieter: Anmelden zum Anzeigen
Antigen
  • AW259666
  • pp60c-src
  • C-src1
  • CG7524
  • D-Src64B
  • D-src
  • DSRC64
  • DSrc
  • DSrc64
  • DSrc64B
  • Dm SRC1
  • DmHD-358
  • Dmel\CG7524
  • Dsrc
  • Dsrc64
  • Dsrc64B
  • HD-358
  • MRE5
  • SRC 64B
  • Src
  • Src1
  • Src64
  • c-src
  • dSrc
  • dsrc
  • src
  • src1
  • src64
  • src64B
  • src64b
  • ASV
  • SRC1
  • c-SRC
  • p60-Src
  • c-Src
  • PP60C-SCR
  • SDR
  • SRC proto-oncogene, non-receptor tyrosine kinase
  • uncharacterized LOC5574802
  • proto-oncogene tyrosine-protein kinase src
  • Rous sarcoma oncogene
  • Src oncogene at 64B
  • SRC
  • LOC5574802
  • CpipJ_CPIJ010659
  • Src
  • Src64B
Epitop
pTyr418
51
36
34
34
25
22
19
15
15
12
11
11
7
7
7
6
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Human, Maus, Ratte (Rattus)
453
260
210
26
16
16
15
12
11
8
7
6
5
4
4
1
1
1
1
1
1
Wirt
Maus
402
72
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser SRC Antikörper ist konjugiert mit Alexa Fluor 488
20
19
15
15
14
13
3
2
2
2
2
2
2
2
2
2
2
2
1
Applikation
Intracellular Staining (ICS)
414
240
192
58
50
30
27
22
20
14
12
5
3
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Phosflow™
Immunogen Phosphorylated Human Src Peptide
Klon K98-37
Isotyp IgG1, kappa
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmide, Primers & weitere SRC products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Src (SRC Antibody Abstract)
Hintergrund The non-receptor protein tyrosine kinases of the Src family have similar domain structures with considerable sequence homologies. They are associated with cell membranes and are activated by a wide variety of cell-surface receptors. Through their participation in cell-signaling cascades that mediate an array of cellular responses, they control essential cellular activities. Src, the prototype of the Src family, is encoded by the SRC protooncogene, which is a homolog of the v-Src gene of the Rous sarcoma virus. Src’s oncogenic potential may be a consequence of its role as a regulator of cell growth, morphology, motility, and adhesion. The protein contains domains that mediate protein-protein interactions and regulation of its tyrosine kinase activity. Mechanisms that regulate Src activity include phosphorylations of serines and tyrosines in most of the domains and interactions with receptor proteins, integrins, and other binding proteins. In particular, autophosphorylation at tyrosine 418 (Y418) activates tyrosine kinase activity. Phosphorylations at other sites are mediated by kinases such as cdc2, CSK, and CHK in signaling cascades and may up- or down-regulate Src activity. The K98-37 monoclonal antibody recognizes the phosphorylated Y418 in the protein kinase domain of activated Src. The orthologous phosphorylation sites in chicken, mouse, and rat Src are Y415, Y423, and Y418, respectively. Because of sequence similarities in this region, it may also recognize the homologous sites of some other Src family members: Lyn (pY396), Fyn (pY420), Hck (pY410), Lck (pY394), and Yes (pY425).
Synonyms: c-Src, p60-Src, SRC, SRC1
Pathways JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikations-hinweise Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Produkt verwendet in: Alvarez, Kantarjian, Cortes: "The role of Src in solid and hematologic malignancies: development of new-generation Src inhibitors." in: Cancer, Vol. 107, Issue 8, pp. 1918-29, 2006 (PubMed).

Frame: "Newest findings on the oldest oncogene; how activated src does it." in: Journal of cell science, Vol. 117, Issue Pt 7, pp. 989-98, 2004 (PubMed).

Arias-Salgado, Lizano, Sarkar, Brugge, Ginsberg, Shattil: "Src kinase activation by direct interaction with the integrin beta cytoplasmic domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 23, pp. 13298-302, 2003 (PubMed).

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