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LAT Antikörper (pTyr226) (Alexa Fluor 488)

LAT Reaktivität: Human ICS Wirt: Maus Monoclonal J96-1238-58-93 Alexa Fluor 488
Produktnummer ABIN1177082
  • Target Alle LAT Antikörper anzeigen
    LAT (Linker For Activation of T Cells (LAT))
    Bindungsspezifität
    • 19
    • 15
    • 15
    • 8
    • 8
    • 7
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr226
    Reaktivität
    • 134
    • 59
    • 41
    • 1
    • 1
    Human
    Wirt
    • 117
    • 17
    • 2
    • 1
    Maus
    Klonalität
    • 119
    • 18
    Monoklonal
    Konjugat
    • 66
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Dieser LAT Antikörper ist konjugiert mit Alexa Fluor 488
    Applikation
    • 75
    • 41
    • 39
    • 39
    • 18
    • 12
    • 11
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Intracellular Staining (ICS)
    Marke
    BD Phosflow™
    Aufreinigung
    Purified from tissue culture supernatant or ascites by affinity chromatography
    Immunogen
    Phosphorylated Human LAT Peptide
    Klon
    J96-1238-58-93
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product LAT Primärantikörper
  • Probenmenge
    20 μL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

    Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, (2002) (PubMed).

    Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, (2002) (PubMed).

    Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, (2001) (PubMed).

    Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, (2001) (PubMed).

  • Target
    LAT (Linker For Activation of T Cells (LAT))
    Andere Bezeichnung
    LAT (LAT Produkte)
    Synonyme
    LAT antikoerper, lat antikoerper, LAT1 antikoerper, pp36 antikoerper, linker for activation of T-cells antikoerper, linker for activation of T cells antikoerper, LAT antikoerper, lat antikoerper, Lat antikoerper
    Hintergrund
    Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development. The J96-1238.58.93 monoclonal antibody recognizes the phosphorylated tyrosine 226 (pY226) of LAT, which is one of the phosphotyrosine sites required for binding Vav, Grb2, and Gads.
    Pathways
    T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung
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