IL1R1 Antikörper

Produktnummer ABIN1176949

Produktdetails anti-IL1R1 Antikörper

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL1R1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Reagent (BR)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2

Klon: 35F5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-IL1R1 Antikörper (ABIN1176949)

McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).

Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, (1989) (PubMed).

Details zu IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Andere Bezeichnung: CD121a (IL1R1 Produkte)

Synonyme: CD121A antikoerper, D2S1473 antikoerper, IL-1R-alpha antikoerper, IL1R antikoerper, IL1RA antikoerper, P80 antikoerper, CD121a antikoerper, CD121b antikoerper, IL-iR antikoerper, Il1r-1 antikoerper, DER4 antikoerper, Fit-1 antikoerper, Ly84 antikoerper, ST2L antikoerper, St2 antikoerper, St2-rs1 antikoerper, T1 antikoerper, T1/ST2 antikoerper, interleukin 1 receptor type 1 antikoerper, interleukin 1 receptor, type I antikoerper, interleukin 1 receptor-like 1 antikoerper, IL1R1 antikoerper, Il1r1 antikoerper, Il1rl1 antikoerper

Hintergrund: The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80

Pathways: NF-kappaB Signalweg, Carbohydrate Homeostasis, Cancer Immune Checkpoints