IL1R1 Antikörper (Interleukin 1 Receptor, Type I)

Details for Product anti-IL1R1 Antibody No. ABIN1176949
Antigen
  • CD121A
  • D2S1473
  • IL-1R-alpha
  • IL1R
  • IL1RA
  • P80
  • CD121a
  • CD121b
  • IL-iR
  • Il1r-1
  • interleukin 1 receptor type 1
  • interleukin 1 receptor, type I
  • IL1R1
  • Il1r1
Reaktivität
Maus
166
88
52
2
2
2
2
1
1
1
1
Wirt
Ratte
162
32
31
9
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser IL1R1 Antikörper ist unkonjugiert
19
12
8
8
7
5
3
3
2
2
2
2
1
1
1
1
1
1
1
Applikation
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
153
109
69
36
26
17
15
14
11
8
5
4
4
2
2
2
2
2
2
1
1
1
Optionen
Marke BD Pharmingen™
Immunogen IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
Klon 35F5
Isotyp IgG1, kappa
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität 0.2 μm filtered
Endotoxin-Niveau Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Plasmids, Primers & others Plasmide, Primers & weitere IL1R1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CD121a (IL1R1 Antibody Abstract)
Hintergrund The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80
Pathways NF-kappaB Signalweg, Carbohydrate Homeostasis, Cancer Immune Checkpoints
Applikations-hinweise Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Konservierungs-mittel Azide free
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Produkt verwendet in: McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, 1991 (PubMed).

Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, 1989 (PubMed).

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