Kaninchen anti-Human IgG1 Antikörper

Produktnummer ABIN1172190

Produktdetails

Target: IgG1

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Spezifität: The antibody is a rabbit polyclonal antibody raised against IgG1. It has been selected for its ability to recognize IgG1 in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography

Immunogen: Native Protein IgG1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

• Western blotting: 0.5-2 μg/mL
• Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
• Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
• Immunohistochemistry in paraffin section: 5-20 μg/mL
• Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
• Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Referenzen

Shao, Fu, Liu, Wang, Ding, Wang, Li, Shao: "IgG autoantibody subclasses altered in immuno-related hemocytopenia." in: Cellular immunology, Vol. 294, Issue 1, pp. 13-20, (2015) (PubMed).

Antigendetails

Target: IgG1

Abstract: IgG1 Produkte

Substanzklasse: Antibody

Hintergrund: Alternative Names: IGHG1, Ig Gamma-1 Chain C Region, Immunoglobulin Heavy Constant Gamma 1, G1m Marker, Immunoglobulin Gm1