Ziege anti-Frettchen IgA (Chain alpha) Antikörper (HRP)

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Antigen
Epitop
Chain alpha
270
143
128
50
39
19
16
16
16
6
6
3
3
2
2
2
2
2
1
1
1
1
Reaktivität
Frettchen
782
210
125
86
52
49
40
39
38
33
29
22
14
12
6
5
4
3
3
3
2
1
1
1
Wirt
Ziege
770
329
294
42
40
18
9
Klonalität
Polyklonal
Konjugat
HRP
193
190
150
89
58
39
29
24
15
12
12
12
10
9
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Ferret IgA alpha (alpha chain).
Reinigung Prepared from monospecific antiserum by Immunoaffinity Chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Hintergrund Synonyms: Ferret Immunoglobulin A
Applikationshinweise Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and protease free) as stabilizer and 0.01 % (w/v) thimerosal as preservative.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen Biohazard Informations: This product contains thimerosal which is hazardous.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Allgemeine Veröffentlichungen Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).