Avidin Antikörper (HRP)

Details for Product anti-Avidin Antibody No. ABIN116963, Anbieter: Anmelden zum Anzeigen
Antigen
  • avidin
  • avd
  • LOC723980
  • AVD
Reaktivität
Huhn
32
26
10
9
8
Wirt
Kaninchen
43
35
2
Klonalität
Polyklonal
Konjugat
Dieser Avidin Antikörper ist konjugiert mit HRP
11
10
9
4
4
4
4
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
70
69
58
10
9
8
8
5
4
4
3
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Avidin [Hen Egg White].
Spezifität This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown.
Reinigung Immunoaffinity chromatography.
Hintergrund The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
Gen-ID 396260
NCBI Accession NP_990651
UniProt P02701
Applikationshinweise Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: This product has been assayed against 1.0 μg of Avidin [Hen EggWhite ] in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,000 to 1: 27,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 2.0 mL of deionized water (or equivalent).
Konzentration 10.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
Konservierungsmittel Gentamicin sulfate
Vorsichtsmaßnahmen This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Allgemeine Veröffentlichungen Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).

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