NLRP3 Antikörper (Pyrin Domain)

Produktnummer ABIN1169100

Produktdetails anti-NLRP3 Antikörper

Highlights:

• NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
• This un-conjugated monoclonal antibody has 60 PubMed citations

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Bindungsspezifität: AA 1-93, Pyrin Domain

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Spezifität: Recognizes mouse and human NLRP3/NALP3.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from concentrated hybridoma tissue culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).

Klon: Cryo-2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-NLRP3 Antikörper (ABIN1169100)

Zhu, Tang: "LncRNA Gm14205 induces astrocytic NLRP3 inflammasome activation via inhibiting oxytocin receptor in postpartum depression." in: Bioscience reports, Vol. 40, Issue 8, (2020) (PubMed).

Wong, Lewis, Doherty, Shi, Cashikar, Amelianchik, Tymchuk, Sullivan, Qian, Covey, Petsko, Holtzman, Paul, Luo: "25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner." in: Journal of neuroinflammation, Vol. 17, Issue 1, pp. 192, (2020) (PubMed).

Hoyle, Redondo-Castro, Cook, Tzeng, Allan, Brough, Lemarchand: "Hallmarks of NLRP3 inflammasome activation are observed in organotypic hippocampal slice culture." in: Immunology, Vol. 161, Issue 1, pp. 39-52, (2020) (PubMed).

Shrivastava, Visoso-Carvajal, Garcia-Cordero, Leon-Juarez, Chavez-Munguia, Lopez, Nava, Villegas-Sepulveda, Cedillo-Barron: "Dengue Virus Serotype 2 and Its Non-Structural Proteins 2A and 2B Activate NLRP3 Inflammasome." in: Frontiers in immunology, Vol. 11, pp. 352, (2020) (PubMed).

Wang, Hu, Wu, Feng, Li, Liu, Wang, Chen, Tian, Xiao, Zhang, Shereen, Chen, Pan, Wan, Wu, Wu: "STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection." in: PLoS pathogens, Vol. 16, Issue 3, pp. e1008335, (2020) (PubMed).

Lai, Hsu, Lee, Chiang: "Microtubule-Mediated NLRP3 Inflammasome Activation Is Independent of Microtubule-Associated Innate Immune Factor GEF-H1 in Murine Macrophages." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Wooff, Fernando, Wong, Dietrich, Aggio-Bruce, Chu-Tan, Robertson, Doyle, Man, Natoli: "Caspase-1-dependent inflammasomes mediate photoreceptor cell death in photo-oxidative damage-induced retinal degeneration." in: Scientific reports, Vol. 10, Issue 1, pp. 2263, (2020) (PubMed).

Svensson, Andersson, Manouchehrian, Yang, Deierborg: "Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease." in: Scientific reports, Vol. 10, Issue 1, pp. 1346, (2020) (PubMed).

Cai, Ge, Mennone, Hoque, Ouyang, Boyer: "Inflammasome Is Activated in the Liver of Cholestatic Patients and Aggravates Hepatic Injury in Bile Duct-Ligated Mouse." in: Cellular and molecular gastroenterology and hepatology, Vol. 9, Issue 4, pp. 679-688, (2020) (PubMed).

Dionísio, Oliveira, Gaspar, Gama, Castro-Caldas, Amaral, Rodrigues: "Ablation of RIP3 protects from dopaminergic neurodegeneration in experimental Parkinson's disease." in: Cell death & disease, Vol. 10, Issue 11, pp. 840, (2020) (PubMed).

Du, Lu, Wang, Ding, Wu, Hu: "The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3." in: Experimental & molecular medicine, Vol. 51, Issue 8, pp. 1-13, (2020) (PubMed).

Hoss, Mueller, Rojas Ringeling, Rodriguez-Alcazar, Brinkschulte, Seifert, Stahl, Broderick, Putnam, Kolodner, Canzar, Geyer, Hoffman, Latz: "Alternative splicing regulates stochastic NLRP3 activity." in: Nature communications, Vol. 10, Issue 1, pp. 3238, (2020) (PubMed).

Sharif, Wang, Wang, Magupalli, Andreeva, Qiao, Hauenstein, Wu, Núñez, Mao, Wu: "Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome." in: Nature, Vol. 570, Issue 7761, pp. 338-343, (2020) (PubMed).

Xue, Xi, Liu, Guo, Zhang, Zhang, Li, Yang, Zhou, Yang, Zhang, Zhang, Gu, Yang, Da, Yao, Duo, Zhang: "miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock." in: Cell death & disease, Vol. 10, Issue 6, pp. 461, (2020) (PubMed).

Huang, Feng, Jiang, Li, Xiang, Guo, Yang, Fei, Guo, Zheng, Wu, Chen: "VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages." in: Science advances, Vol. 5, Issue 1, pp. eaau7426, (2020) (PubMed).

Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu: "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting: "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR." in: The Journal of experimental medicine, Vol. 216, Issue 12, pp. 2838-2853, (2020) (PubMed).

Sanchez-Lopez, Zhong, Stubelius, Sweeney, Booshehri, Antonucci, Liu-Bryan, Lodi, Terkeltaub, Lacal, Murphy, Hoffman, Tiziani, Guma, Karin: "Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production." in: Cell metabolism, Vol. 29, Issue 6, pp. 1350-1362.e7, (2020) (PubMed).

Kiyohara, Sujino, Teratani, Miyamoto, Arai, Nomura, Harada, Aoki, Koda, Mikami, Mizuno, Naganuma, Hisamatsu, Kanai: "Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 1, pp. 135-156, (2019) (PubMed).

Hafner-Bratkovič, Sušjan, Lainšček, Tapia-Abellán, Cerović, Kadunc, Angosto-Bazarra, Pelegrin, Jerala: "NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway." in: Nature communications, Vol. 9, Issue 1, pp. 5182, (2019) (PubMed).

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3/NALP3 (NLRP3 Produkte)

Synonyme: CIAS1 antikoerper, Nalp3 antikoerper, avp antikoerper, cias1 antikoerper, nalp3 antikoerper, pypaf1 antikoerper, agtavprl antikoerper, AGTAVPRL antikoerper, AII antikoerper, AVP antikoerper, C1orf7 antikoerper, CLR1.1 antikoerper, FCAS antikoerper, FCU antikoerper, MWS antikoerper, NALP3 antikoerper, PYPAF1 antikoerper, AII/AVP antikoerper, Cias1 antikoerper, Mmig1 antikoerper, Pypaf1 antikoerper, NLR family pyrin domain containing-like antikoerper, NLR family pyrin domain containing 3 antikoerper, NLR family, pyrin domain containing 3 L homeolog antikoerper, NLR family, pyrin domain containing 3 antikoerper, NLRPL antikoerper, NLRP3 antikoerper, nlrp3.L antikoerper, Nlrp3 antikoerper

Hintergrund: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

UniProt: Q8R4B8

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome