GFP Antikörper (Green Fluorescent Protein) (Texas Red (TR))

Details for Product anti-GFP Antibody No. ABIN116751, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
632
19
5
5
2
2
1
1
Wirt
Ziege
335
172
80
43
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist konjugiert mit Texas Red (TR)
33
30
28
14
13
12
11
10
9
9
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6
6
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6
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4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
535
355
203
163
140
136
97
85
74
25
15
13
12
8
7
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6
5
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3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Spezifität Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Produktmerkmale Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.2 moles Texas Red per mole of Goat IgG.
Reinigung Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
Gen-ID 6100
UniProt P42212
Applikationshinweise Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigenrecognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 witn 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Produkt verwendet in: Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, 2012 (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, 2009 (PubMed).

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