GDNF
Reaktivität: Human
WB, IHC, ICC, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: To detect hGDNF by direct ELISA (using 100 ml/well antibody solution) this GDNFantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthGDNF. Western Blot: To detect hGDNF by Western Blot analysis this GDNF antibody can be used ata concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Centrifuge vial prior to opening! This product is photosensitive and should be protected from light
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C. DO NOT FREEZE!
Target
GDNF
(Glial Cell Line Derived Neurotrophic Factor (GDNF))
Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement.Synonyms: ATF, Astrocyte-derived trophic factor, Glial cell line-derived neurotrophic factor