E2F4 Antikörper (E2F Transcription Factor 4, P107/p130-Binding)

Details for Product anti-E2F4 Antibody No. ABIN115206, Anbieter: Anmelden zum Anzeigen
Antigen
  • E2F-4
  • 2010111M04Rik
  • AI427446
  • E2F4
  • e2f-4
  • fb72f07
  • wu:fb72f07
  • wu:fe05f06
  • zgc:63815
  • E2F transcription factor 4
  • E2F transcription factor 4 S homeolog
  • E2F4
  • E2f4
  • e2f4.S
  • e2f4
Reaktivität
Human, Ratte (Rattus)
94
40
36
8
7
6
6
6
4
3
3
2
1
Wirt
Maus
45
29
23
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser E2F4 Antikörper ist unkonjugiert
5
4
3
3
3
3
Applikation
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
52
22
19
15
10
5
3
2
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen BALB/C mice were immunized with a recombinant human E2F-4 protein.
Klon 4E2F04
Isotyp IgG1
Spezifität This antibody reacts with a protein of 56 kDa, E2F4.
Cellular Localization: Nuclear, cytoplasmic.
Reinigung Protein G purified ascites
Andere Bezeichnung E2F4 (E2F4 Antibody Abstract)
Hintergrund E2F's are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.Synonyms: E2F transcription factor 4, E2F-4, Transcription factor E2F4, p107/p130-binding
Gen-ID 1874
NCBI Accession NP_001941
UniProt Q16254
Pathways Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size
Applikationshinweise Immunofluorescence. Immunohistochemistry on paraffin embedded sections: 1: 25-1: 50 inan ABC method 30 minutes at room temperature. Formalin fixed paraffin embedded tissuesections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0prior to immunostaining. Western Blot (1: 50 - 1: 100). Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS, containing sodium azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN115206) Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 Ab using peroxidase...
Allgemeine Veröffentlichungen Ikeda, Jakoi, Nevins: "A unique role for the Rb protein in controlling E2F accumulation during cell growth and differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3215-20, 1996 (PubMed).

Johnson, Cress, Jakoi, Nevins: "Oncogenic capacity of the E2F1 gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 26, pp. 12823-7, 1995 (PubMed).

Krek, Livingston, Shirodkar: "Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members." in: Science (New York, N.Y.), Vol. 262, Issue 5139, pp. 1557-60, 1993 (PubMed).

Haben Sie etwas anderes gesucht?