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CD44 Antikörper (FITC)

CD44 Reaktivität: Ratte FACS, IHC (p), IHC (fro) Wirt: Maus Monoclonal OX-49 FITC
Produktnummer ABIN114364
  • Target Alle CD44 Antikörper anzeigen
    CD44
    Reaktivität
    • 310
    • 170
    • 81
    • 30
    • 25
    • 24
    • 16
    • 11
    • 9
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    • 8
    • 6
    • 5
    • 4
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    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Wirt
    • 172
    • 123
    • 95
    • 2
    • 1
    Maus
    Klonalität
    • 275
    • 116
    • 2
    Monoklonal
    Konjugat
    • 172
    • 44
    • 31
    • 27
    • 19
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD44 Antikörper ist konjugiert mit FITC
    Applikation
    • 275
    • 157
    • 94
    • 77
    • 72
    • 72
    • 62
    • 40
    • 38
    • 31
    • 30
    • 13
    • 9
    • 7
    • 7
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    • 6
    • 5
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    • 4
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    • 3
    • 2
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    • 2
    • 2
    • 2
    • 1
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    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This anti-Rat CD44 monoclonal antibody recognizes an epitope on both standard CD44 and its splice variant.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Rat
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    T cell blasts.
    Klon
    OX-49
    Isotyp
    IgG2a
    Top Product
    Discover our top product CD44 Primärantikörper
  • Applikationshinweise
    Suitable for Flow cytometry (See protocol) and Immunohistochemistry on frozen andparaffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1. 0-0. 5 µg* of ABIN114364 or ABIN114364X. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Rat Strain: WistarCell Concentration: 1 x 10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: FITC Mouse IgG2a. Cell Source Percentage of cells stained above control: Thymus: 99. 6%Spleen: 75. 9%Lymph Node: 96. 8%N. B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigatordetermine dilutions appropriate for individual use.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizer
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD44
    Andere Bezeichnung
    CD44 (CD44 Produkte)
    Synonyme
    CDW44 antikoerper, CSPG8 antikoerper, ECMR-III antikoerper, HCELL antikoerper, HUTCH-I antikoerper, IN antikoerper, LHR antikoerper, MC56 antikoerper, MDU2 antikoerper, MDU3 antikoerper, MIC4 antikoerper, Pgp1 antikoerper, AU023126 antikoerper, AW121933 antikoerper, AW146109 antikoerper, HERMES antikoerper, Ly-24 antikoerper, Pgp-1 antikoerper, CD44A antikoerper, METAA antikoerper, RHAMM antikoerper, XCD44 antikoerper, cd44 antikoerper, cdw44 antikoerper, cspg8 antikoerper, ecmr-iii antikoerper, hcell antikoerper, lhr antikoerper, mc56 antikoerper, mdu2 antikoerper, mdu3 antikoerper, mic4 antikoerper, mutch-i antikoerper, pgp1 antikoerper, CD44 molecule (Indian blood group) antikoerper, syndecan 4 antikoerper, CD44 antigen antikoerper, CD44 molecule (Indian blood group) S homeolog antikoerper, CD44 antikoerper, SDC4 antikoerper, Cd44 antikoerper, cd44.S antikoerper
    Hintergrund
    This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
    Gen-ID
    10116
    UniProt
    P26051
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling
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