Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD11c Antikörper (APC)

ITGAX Reaktivität: Maus FACS, IF Wirt: Armenischer Hamster Monoclonal N418 APC
Produktnummer ABIN1112431
  • Target Alle CD11c (ITGAX) Antikörper anzeigen
    CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
    Reaktivität
    • 178
    • 71
    • 13
    • 12
    • 10
    • 9
    • 9
    • 5
    • 1
    Maus
    Wirt
    • 129
    • 52
    • 29
    • 19
    • 5
    Armenischer Hamster
    Klonalität
    • 185
    • 44
    • 2
    Monoklonal
    Konjugat
    • 80
    • 18
    • 17
    • 17
    • 12
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD11c Antikörper ist konjugiert mit APC
    Applikation
    • 142
    • 52
    • 51
    • 50
    • 40
    • 34
    • 20
    • 13
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Produktmerkmale
    Monoclonal Hamster Anti-Mouse CD11c APC is recommended for use in flow cytometry for identification of the Mouse CD11c.
    Immunogen
    Murine spleen dendritic cells
    Klon
    N418
    Isotyp
    IgG
    Top Product
    Discover our top product ITGAX Primärantikörper
  • Applikationshinweise
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. Recommeded Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometry analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal tless or equal to 0.25 µg per 10^6 cells in 100 µL volume or 100 µL of whole bloodIt is recommended that the reagent be titrated for optimal performance for each application.
    Kommentare

    Allophycocianin (Abs/ Em: 651/ 662 nm).

    Aufbereitung der Reagenzien
    1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add antibody and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The conjugate is provided Phosphate-buffered solution, pH 7.2, containing 0.09% Sodium azide and 0.2% (w/v) BSA (Sigma).
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Lagerung
    4 °C
  • Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).

  • Target
    CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
    Andere Bezeichnung
    CD11c (ITGAX Produkte)
    Synonyme
    CD11C antikoerper, SLEB6 antikoerper, RGD1561123 antikoerper, AI449405 antikoerper, Cd11c antikoerper, Cr4 antikoerper, N418 antikoerper, integrin subunit alpha X antikoerper, integrin alpha X antikoerper, ITGAX antikoerper, Itgax antikoerper
    Hintergrund
    CD11c is a 150 kDa glycoprotein, also known as alphaX integrin, CR4, or p150. CD11c forms a alphaXbeta2heterodimer with beta2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The alphaXbeta2 integrin plays an important role in cell-cell contact by binding its ligands, iC3b, fibrinogen, and CD54.
    Pathways
    Komplementsystem, Activated T Cell Proliferation, Integrin Complex
Sie sind hier:
Kundenservice