CD99 Antikörper (APC)

Produktnummer ABIN1112237

Produktdetails anti-CD99 Antikörper (APC)

Target: CD99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Mouse Anti-Human CD99 APC, is recommended for use in flow cytometry for identification of human leukocyte lineages.

Klon: HI156

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Allophycocyanin (Febico, Far East Bio-Tech Co.).

Probennahme: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD99 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Referenzen für anti-CD99 Antikörper (APC) (ABIN1112237)

Sebire, Gibson, Rampling, Williams, Malone, Ramsay: "Immunohistochemical findings in embryonal small round cell tumors with molecular diagnostic confirmation." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 13, Issue 1, pp. 1-5, (2005) (PubMed).

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99 (CD99 Produkte)

Synonyme: 1110061M03Rik antikoerper, 2410026K10Rik antikoerper, D4 antikoerper, Pilr-l antikoerper, pilr-1 antikoerper, HBA71 antikoerper, MIC2 antikoerper, MIC2X antikoerper, MIC2Y antikoerper, MSK5X antikoerper, CD99 antigen antikoerper, CD99 molecule (Xg blood group) antikoerper, CD99 molecule antikoerper, Cd99 antikoerper, CD99 antikoerper

Substanzklasse: Viral Protein

Hintergrund: This antibody recognizes the MIC 2 (E2) antigen expressed on most human cells except those of the myeloid family. The 32kDa molecule on thymocytes and T cells is involved in rosette formation.