CD11c Antikörper (PerCP)

Produktnummer ABIN1112052

Produktdetails anti-CD11c Antikörper (PerCP)

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD11c Antikörper ist konjugiert mit PerCP

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Mouse Anti-Human CD11c PerCP, is recommended for use in flow cytometry for identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.

Klon: BU-15

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Peridin-cholophyll-protein complex (Prozyme).

Probennahme: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 20 µl of CD11c PerCP and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4 °C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 20ml/test

Buffer: The conjugate is provided in liquid form in buffer containing Stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Referenzen für anti-CD11c Antikörper (PerCP) (ABIN1112052)

Sánchez, Almeida, Vidriales, López-Berges, García-Marcos, Moro, Corrales, Calmuntia, San Miguel, Orfao: "Incidence of phenotypic aberrations in a series of 467 patients with B chronic lymphoproliferative disorders: basis for the design of specific four-color stainings to be used for minimal residual disease investigation." in: Leukemia, Vol. 16, Issue 8, pp. 1460-9, (2002) (PubMed).

Details zu CD11c

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Andere Bezeichnung: CD11c (ITGAX Produkte)

Synonyme: CD11C antikoerper, SLEB6 antikoerper, RGD1561123 antikoerper, AI449405 antikoerper, Cd11c antikoerper, Cr4 antikoerper, N418 antikoerper, integrin subunit alpha X antikoerper, integrin alpha X antikoerper, ITGAX antikoerper, Itgax antikoerper

Hintergrund: This antibody reacts with the X chain of the CD11c/CD18 integrin heterodimer expressed on monocytes and weakly on granulocytes. The antibody stains macrophages in most types of tissues. flow cytometry the antibody labels a T-cell subset and weakly a B-cell subset.

Pathways: Komplementsystem, Activated T Cell Proliferation, Integrin Complex