Applikations-hinweise |
Immunohistochemistry on Frozen Sections: 5-10 μg/mL (1/40-1/80). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/20). Proteinase K treatment forantigen retrieval is recommended. Recommended Positive Control: Human cortex. Has been described to work in ELISA and Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
Protokoll | Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer’s hemalum |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
Rekonstitution | Restore in 0.5 mL distilled water. |
Konzentration | 0.4 mg/mL |
Buffer | Stock solution contains PBS pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer. |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handhabung | This product is photosensitive and should be protected from light |
Lagerung | 4 °C |
Informationen zur Lagerung | Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! |
Produkt verwendet in: |
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Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed). Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed). Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed). Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed). Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed). |