Muscarinic Acetylcholine Receptor M2 Antikörper (Extracellular Loop)
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- Target Alle Muscarinic Acetylcholine Receptor M2 (CHRM2) Antikörper anzeigen
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
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Bindungsspezifität
- AA 168-192, Extracellular Loop
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Reaktivität
- Human, Maus, Ratte, Meerschweinchen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Muscarinic Acetylcholine Receptor M2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Sequenz
- VRTVEDGECY IQFFSNAAVT FGTAI(C)
- Spezifität
- The monoclonal antibody B8E5 recognizes Human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the Human M2 receptor.
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human, Guinea pig, Mouse, Rat.
- Aufreinigung
- Protein G Chromatography
- Immunogen
- Human second extracellular loop residues 168-192
- Klon
- B8E5
- Isotyp
- IgG2a
- Top Product
- Discover our top product CHRM2 Primärantikörper
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.1 mg/mL
- Buffer
- PBS, 0.1 % BSA
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C.
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- Target
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
- Andere Bezeichnung
- Muscarinic Acetylcholine Receptor M2 (CHRM2 Produkte)
- Synonyme
- HM2 antikoerper, AChR-M2 antikoerper, Chrm-2 antikoerper, M2 antikoerper, chrm2 antikoerper, Acm2 antikoerper, GPM2 antikoerper, cholinergic receptor muscarinic 2 antikoerper, cholinergic receptor, muscarinic 2, cardiac antikoerper, cholinergic receptor, muscarinic 2a antikoerper, cholinergic receptor, muscarinic 2 antikoerper, CHRM2 antikoerper, Chrm2 antikoerper, chrm2a antikoerper
- Hintergrund
- This receptor is an integral membrane protein consisting of seven membrane spanning a-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.Synonyms: CHRM2, mAChR M2, mAChR-M2
- Gen-ID
- 1129
- NCBI Accession
- NP_000730
- UniProt
- P08172
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