E-cadherin Antikörper

Produktnummer ABIN1106274

Produktdetails anti-E-cadherin Antikörper

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E-cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The antibody 5H9 recognizes both the 120 kDa E-Cadherin and its 80 kDa trypsin-resistant extracellular part.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Purified

Immunogen: Human Uvomorulin

Klon: 5H9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 0.09 % Sodium Azide

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-E-cadherin Antikörper (ABIN1106274)

Schwechheimer, Zhou, Birchmeier: "E-Cadherin in human brain tumours: loss of immunoreactivity in malignant meningiomas." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 2, pp. 163-7, (1998) (PubMed).

Mackie, Campbell, Turbitt: "Use of NK1 C3 monoclonal antibody in the assessment of benign and malignant melanocytic lesions." in: Journal of clinical pathology, Vol. 37, Issue 4, pp. 367-72, (1984) (PubMed).

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: CD324 / Cadherin-1 (CDH1 Produkte)

Synonyme: Arc-1 antikoerper, CD324 antikoerper, CDHE antikoerper, ECAD antikoerper, LCAM antikoerper, UVO antikoerper, E-cadherin antikoerper, cdh1 antikoerper, si:dz180o5.2 antikoerper, uvo antikoerper, lcam antikoerper, E-Cad antikoerper, XBcad antikoerper, l-cam antikoerper, xcdh1 antikoerper, cdhc-A antikoerper, xb-cad antikoerper, XTCAD-1 antikoerper, c-cadherin antikoerper, cadherin-1 antikoerper, uvomorulin antikoerper, XB-cadherin antikoerper, arc-1 antikoerper, cd324 antikoerper, cdhe antikoerper, ecad antikoerper, AA960649 antikoerper, E-cad antikoerper, Ecad antikoerper, L-CAM antikoerper, Um antikoerper, cadherin 1 antikoerper, si:busm1-180o5.2 antikoerper, cadherin 1, type 1, E-cadherin (epithelial) antikoerper, cadherin 1, type 1 S homeolog antikoerper, CDH1 antikoerper, si:busm1-180o5.2 antikoerper, cdh1 antikoerper, Cdh1 antikoerper, cdh1.S antikoerper

Hintergrund: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin αEβ7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia. One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, ECadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90 % of the malignant tumors are carcinomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-Cadherin expression. Therefore, the expression of E-Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

Gen-ID: 999

NCBI Accession: NP_004351

UniProt: P12830

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation