His Tag Antikörper

Details zu Produkt Nr. ABIN1105141, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
624
28
15
4
4
1
Wirt
Maus
413
188
27
23
8
8
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
58
46
37
17
13
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
568
406
164
107
83
82
78
27
13
11
8
7
6
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
Sequenz HHHHHH
Klon HIS-H8
Isotyp IgG2b
Reinigung Affinity Chromatography.
Andere Bezeichnung 6xHistidine Epitope Tag
Substanzklasse Tag
Hintergrund Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store the antibody (in aliquots) at -20 °C.
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody
Immunofluorescence (IF) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody (Image 2)
Allgemeine Veröffentlichungen Xu, Liu, He: "Expression and purification of soluble human programmed death-1 in Escherichia coli." in: Cellular & molecular immunology, Vol. 3, Issue 2, pp. 139-43, 2006 (PubMed).

Terpe: "Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems." in: Applied microbiology and biotechnology, Vol. 60, Issue 5, pp. 523-33, 2003 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

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