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His Tag Antikörper

WB, IP, IF Wirt: Maus Monoclonal HIS-H8 unconjugated
Produktnummer ABIN1105141
  • Target Alle His Tag Antikörper anzeigen
    His Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 94
    • 56
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    Maus
    Klonalität
    • 107
    • 69
    • 10
    Monoklonal
    Konjugat
    • 91
    • 23
    • 14
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser His Tag Antikörper ist unkonjugiert
    Applikation
    • 153
    • 103
    • 38
    • 28
    • 25
    • 17
    • 14
    • 14
    • 14
    • 13
    • 13
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    Sequenz
    HHHHHH
    Aufreinigung
    Affinity Chromatography.
    Immunogen
    Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
    Klon
    HIS-H8
    Isotyp
    IgG2b
    Top Product
    Discover our top product His Tag Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the antibody (in aliquots) at -20 °C.
  • Target
    His Tag
    Andere Bezeichnung
    6xHistidine Epitope Tag (His Tag Produkte)
    Substanzklasse
    Tag
    Hintergrund
    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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