His Tag Antikörper (Biotin)

Details zu Produkt Nr. ABIN1102040, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
624
28
15
4
4
1
Wirt
Maus
413
188
27
23
8
8
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Biotin
58
45
37
17
13
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
568
405
165
108
83
81
78
27
13
11
8
7
6
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Hapten
Sequenz HHHHHH
Klon 33D10-D2
Isotyp IgG
Spezifität This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Reinigung Protein A purified
Substanzklasse Tag
Applikationshinweise Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Rekonstitution deionized water
Konzentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung avoid freeze thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Bilder des Herstellers
 image for anti-His Tag antibody (Biotin) (ABIN1102040) anti-His Tag antibody (Biotin)
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